The odyssey of a regulated transcript

J. Vilardell, P. Chartrand, Robert H. Singer, J. R. Warner

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The transcript of the Saccharomyces cerevisiae gene, RPL30, is subject to regulated splicing and regulated translation, due to a structure that interacts with its own product, ribosomal protein L30. We have followed the fate of the regulated RPL30 transcripts in vivo. Initially, these transcripts abortively enter the splicing pathway, forming an unusually stable association with U1 snRNP. A large proportion of the unspliced molecules, however, are found in the cytoplasm. Most of these are still bound by L30, as only a small fraction are engaged in translation. Eventually, the unspliced RPL30 transcripts escape the grasp of L30, associate with ribosomes, and fall prey to nonsense mediated decay.

Original languageEnglish (US)
Pages (from-to)1773-1780
Number of pages8
JournalRNA
Volume6
Issue number12
DOIs
StatePublished - 2000

Fingerprint

L 30
U1 Small Nuclear Ribonucleoproteins
Hand Strength
Ribosomes
Saccharomyces cerevisiae
Cytoplasm
Genes

Keywords

  • L30
  • mRNA, nonsense mediated decay
  • Nuclear-cytoplasmic transport
  • Polyribosome
  • Regulated splicing
  • Regulated translation
  • Ribosomal protein

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Vilardell, J., Chartrand, P., Singer, R. H., & Warner, J. R. (2000). The odyssey of a regulated transcript. RNA, 6(12), 1773-1780. https://doi.org/10.1017/S135583820000145X

The odyssey of a regulated transcript. / Vilardell, J.; Chartrand, P.; Singer, Robert H.; Warner, J. R.

In: RNA, Vol. 6, No. 12, 2000, p. 1773-1780.

Research output: Contribution to journalArticle

Vilardell, J, Chartrand, P, Singer, RH & Warner, JR 2000, 'The odyssey of a regulated transcript', RNA, vol. 6, no. 12, pp. 1773-1780. https://doi.org/10.1017/S135583820000145X
Vilardell J, Chartrand P, Singer RH, Warner JR. The odyssey of a regulated transcript. RNA. 2000;6(12):1773-1780. https://doi.org/10.1017/S135583820000145X
Vilardell, J. ; Chartrand, P. ; Singer, Robert H. ; Warner, J. R. / The odyssey of a regulated transcript. In: RNA. 2000 ; Vol. 6, No. 12. pp. 1773-1780.
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