The NANIVID: A new device for cancer cell migration studies

Waseem K. Raja, Nathaniel C. Cady, James Castracane, Bojana Gligorijevic, Jacco Van Rheenen, John S. Condeelis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Cancerous tumors are dynamic microenvironments that require unique analytical tools for their study. Better understanding of tumor microenvironments may reveal mechanisms behind tumor progression and generate new strategies for diagnostic marker development, which can be used routinely in histopathological analysis. Previous studies have shown that cell invasion and intravasation are related to metastatic potential and have linked these activities to gene expression patterns seen in migratory and invasive tumor cells in vivo. Existing analytical methods for tumor microenvironments include collection of tumor cells through a catheter needle loaded with a chemical or protein attractant (chemoattractant). This method has some limitations and restrictions, including time constraints of cell collection, long term anesthetization, and in vivo imaging inside the catheter. In this study, a novel implantable device was designed to replace the catheter-based method. The 1.5mm x 0.5mm x 0.24mm device is designed to controllably release chemoattractants for stimulation of tumor cell migration and subsequent cell capture. Devices were fabricated using standard microfabrication techniques and have been shown to mediate controlled release of bovine serum albumin (BSA) and epidermal growth factor (EGF). Optically transparent indium tin oxide (ITO) electrodes have been incorporated into the device for impedance-based measurement of cell density and have been shown to be compatible with in vivo multi-photon imaging of cell migration.

Original languageEnglish (US)
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
DOIs
StatePublished - Dec 1 2008
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI - San Jose, CA, United States
Duration: Jan 21 2008Jan 23 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6859
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
CountryUnited States
CitySan Jose, CA
Period1/21/081/23/08

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Keywords

  • Multi-photon microscopy
  • Poly (ethylene glycol) DiAcrylate (PEGDA)
  • controlled release
  • epidermal growth factor (EGF)
  • fluorescence
  • hydrogel
  • impedance
  • interdigitated electrodes
  • tumor cells

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Raja, W. K., Cady, N. C., Castracane, J., Gligorijevic, B., Van Rheenen, J., & Condeelis, J. S. (2008). The NANIVID: A new device for cancer cell migration studies. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI [68591M] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6859). https://doi.org/10.1117/12.760730