Abstract
Cancerous tumors are dynamic microenvironments that require unique analytical tools for their study. Better understanding of tumor microenvironments may reveal mechanisms behind tumor progression and generate new strategies for diagnostic marker development, which can be used routinely in histopathological analysis. Previous studies have shown that cell invasion and intravasation are related to metastatic potential and have linked these activities to gene expression patterns seen in migratory and invasive tumor cells in vivo. Existing analytical methods for tumor microenvironments include collection of tumor cells through a catheter needle loaded with a chemical or protein attractant (chemoattractant). This method has some limitations and restrictions, including time constraints of cell collection, long term anesthetization, and in vivo imaging inside the catheter. In this study, a novel implantable device was designed to replace the catheter-based method. The 1.5mm x 0.5mm x 0.24mm device is designed to controllably release chemoattractants for stimulation of tumor cell migration and subsequent cell capture. Devices were fabricated using standard microfabrication techniques and have been shown to mediate controlled release of bovine serum albumin (BSA) and epidermal growth factor (EGF). Optically transparent indium tin oxide (ITO) electrodes have been incorporated into the device for impedance-based measurement of cell density and have been shown to be compatible with in vivo multi-photon imaging of cell migration.
Original language | English (US) |
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Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
Volume | 6859 |
DOIs | |
State | Published - 2008 |
Event | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI - San Jose, CA, United States Duration: Jan 21 2008 → Jan 23 2008 |
Other
Other | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI |
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Country | United States |
City | San Jose, CA |
Period | 1/21/08 → 1/23/08 |
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Keywords
- controlled release
- epidermal growth factor (EGF)
- fluorescence
- hydrogel
- impedance
- interdigitated electrodes
- Multi-photon microscopy
- Poly (ethylene glycol) DiAcrylate (PEGDA)
- tumor cells
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
The NANIVID : A new device for cancer cell migration studies. / Raja, Waseem K.; Cady, Nathaniel C.; Castracane, James; Gligorijevic, Bojana; Van Rheenen, Jacco; Condeelis, John S.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6859 2008. 68591M.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - The NANIVID
T2 - A new device for cancer cell migration studies
AU - Raja, Waseem K.
AU - Cady, Nathaniel C.
AU - Castracane, James
AU - Gligorijevic, Bojana
AU - Van Rheenen, Jacco
AU - Condeelis, John S.
PY - 2008
Y1 - 2008
N2 - Cancerous tumors are dynamic microenvironments that require unique analytical tools for their study. Better understanding of tumor microenvironments may reveal mechanisms behind tumor progression and generate new strategies for diagnostic marker development, which can be used routinely in histopathological analysis. Previous studies have shown that cell invasion and intravasation are related to metastatic potential and have linked these activities to gene expression patterns seen in migratory and invasive tumor cells in vivo. Existing analytical methods for tumor microenvironments include collection of tumor cells through a catheter needle loaded with a chemical or protein attractant (chemoattractant). This method has some limitations and restrictions, including time constraints of cell collection, long term anesthetization, and in vivo imaging inside the catheter. In this study, a novel implantable device was designed to replace the catheter-based method. The 1.5mm x 0.5mm x 0.24mm device is designed to controllably release chemoattractants for stimulation of tumor cell migration and subsequent cell capture. Devices were fabricated using standard microfabrication techniques and have been shown to mediate controlled release of bovine serum albumin (BSA) and epidermal growth factor (EGF). Optically transparent indium tin oxide (ITO) electrodes have been incorporated into the device for impedance-based measurement of cell density and have been shown to be compatible with in vivo multi-photon imaging of cell migration.
AB - Cancerous tumors are dynamic microenvironments that require unique analytical tools for their study. Better understanding of tumor microenvironments may reveal mechanisms behind tumor progression and generate new strategies for diagnostic marker development, which can be used routinely in histopathological analysis. Previous studies have shown that cell invasion and intravasation are related to metastatic potential and have linked these activities to gene expression patterns seen in migratory and invasive tumor cells in vivo. Existing analytical methods for tumor microenvironments include collection of tumor cells through a catheter needle loaded with a chemical or protein attractant (chemoattractant). This method has some limitations and restrictions, including time constraints of cell collection, long term anesthetization, and in vivo imaging inside the catheter. In this study, a novel implantable device was designed to replace the catheter-based method. The 1.5mm x 0.5mm x 0.24mm device is designed to controllably release chemoattractants for stimulation of tumor cell migration and subsequent cell capture. Devices were fabricated using standard microfabrication techniques and have been shown to mediate controlled release of bovine serum albumin (BSA) and epidermal growth factor (EGF). Optically transparent indium tin oxide (ITO) electrodes have been incorporated into the device for impedance-based measurement of cell density and have been shown to be compatible with in vivo multi-photon imaging of cell migration.
KW - controlled release
KW - epidermal growth factor (EGF)
KW - fluorescence
KW - hydrogel
KW - impedance
KW - interdigitated electrodes
KW - Multi-photon microscopy
KW - Poly (ethylene glycol) DiAcrylate (PEGDA)
KW - tumor cells
UR - http://www.scopus.com/inward/record.url?scp=78650226874&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650226874&partnerID=8YFLogxK
U2 - 10.1117/12.760730
DO - 10.1117/12.760730
M3 - Conference contribution
AN - SCOPUS:78650226874
SN - 9780819470348
VL - 6859
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -