The murine N-ras gene is not essential for growth and development

Heywood Umanoff, Winfried Edelmann, Angel Pellicer, Raju Kucherlapati

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

The mammalian ras gene family encodes key cell-signaling, cell growth- related proteins that have been highly conserved in species from yeast to man. Specific point mutations in the ras genes are associated with various mammalian tumors. To understand the developmental role of the N-ras protooncogene in the mouse, we have disrupted its gene function by homologous recombination in embryonic stem cells. Mice derived from these cells that are homozygous for the N-ras mutation do not produce any detectable N-Ras protein and are morphologically and histologically indistinguishable from their heterozygous and wild-type siblings. Since N-ras is expressed at high levels in hematopoietic cells, we examined different populations of cells in peripheral blood and found no differences between mutant and normal animals. Our results show that N-ras gene function is dispensable for normal mouse development, growth, and fertility.

Original languageEnglish (US)
Pages (from-to)1709-1713
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number5
DOIs
StatePublished - Feb 28 1995

Fingerprint

ras Genes
Growth and Development
ras Proteins
Homologous Recombination
Embryonic Stem Cells
Point Mutation
Fertility
Siblings
Yeasts
Mutation
Growth
Population
Genes
Neoplasms
Proteins

Keywords

  • embryonic stem cells
  • gene targeting
  • oncogenes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

The murine N-ras gene is not essential for growth and development. / Umanoff, Heywood; Edelmann, Winfried; Pellicer, Angel; Kucherlapati, Raju.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 5, 28.02.1995, p. 1709-1713.

Research output: Contribution to journalArticle

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