The mannose receptor delivers lipoglycan antigens to endosomes for presentation to T cells by CD1b molecules

Theodore I. Prigozy, Peter A. Sieling, Daniel Clemens, Phoebe L. Stewart, Samuel M. Behar, Steven A. Porcelli, Michael B. Brenner, Robert L. Modlin, Mitchell Kronenberg

Research output: Contribution to journalArticle

283 Citations (Scopus)

Abstract

We have characterized the CD1b-mediated presentation pathway for the mycobacterial lipoglycan lipoarabinomannan (LAM) in monocyte-derived antigen-presenting cells. The macrophage mannose receptor (MR) was responsible for uptake of LAM. Antagonism of MR function inhibited both the internalization of LAM and the presentation of this antigen to LAM-reactive T cells. Intracellular MRs were most abundant in early endosomes, but they also were located in the compartment for MHC class II antigen loading (MIIC). Internalized LAM was transported to late endosomes, lysosomes, and MIICs. MRs colocalized with CD1b molecules, suggesting that the MR could deliver LAM to late endosomes for loading onto CD1b. LAM and CD1b colocalized in organelles that may be sites of lipoglycan antigen loading. This pathway links recognition of microbial antigens by a receptor of the innate immune system to the induction of adaptive T cell responses.

Original languageEnglish (US)
Pages (from-to)187-197
Number of pages11
JournalImmunity
Volume6
Issue number2
DOIs
StatePublished - Feb 1997
Externally publishedYes

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Endosomes
Lipopolysaccharides
T-Lymphocytes
Antigens
Antigen Receptors
Histocompatibility Antigens Class II
Antigen Presentation
Antigen-Presenting Cells
Lysosomes
lipoarabinomannan
mannose receptor
Organelles
Monocytes
Immune System
Macrophages

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Prigozy, T. I., Sieling, P. A., Clemens, D., Stewart, P. L., Behar, S. M., Porcelli, S. A., ... Kronenberg, M. (1997). The mannose receptor delivers lipoglycan antigens to endosomes for presentation to T cells by CD1b molecules. Immunity, 6(2), 187-197. https://doi.org/10.1016/S1074-7613(00)80425-2

The mannose receptor delivers lipoglycan antigens to endosomes for presentation to T cells by CD1b molecules. / Prigozy, Theodore I.; Sieling, Peter A.; Clemens, Daniel; Stewart, Phoebe L.; Behar, Samuel M.; Porcelli, Steven A.; Brenner, Michael B.; Modlin, Robert L.; Kronenberg, Mitchell.

In: Immunity, Vol. 6, No. 2, 02.1997, p. 187-197.

Research output: Contribution to journalArticle

Prigozy, TI, Sieling, PA, Clemens, D, Stewart, PL, Behar, SM, Porcelli, SA, Brenner, MB, Modlin, RL & Kronenberg, M 1997, 'The mannose receptor delivers lipoglycan antigens to endosomes for presentation to T cells by CD1b molecules', Immunity, vol. 6, no. 2, pp. 187-197. https://doi.org/10.1016/S1074-7613(00)80425-2
Prigozy, Theodore I. ; Sieling, Peter A. ; Clemens, Daniel ; Stewart, Phoebe L. ; Behar, Samuel M. ; Porcelli, Steven A. ; Brenner, Michael B. ; Modlin, Robert L. ; Kronenberg, Mitchell. / The mannose receptor delivers lipoglycan antigens to endosomes for presentation to T cells by CD1b molecules. In: Immunity. 1997 ; Vol. 6, No. 2. pp. 187-197.
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