The major human papillomavirus protein in cervical cancers is a cytoplasmic phosphoprotein

David Smotkin, F. O. Wettstein

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

In a previous study, the most abundant viral transcript in a human papillomavirus type 16-associated cervical cancer and in a cancer-derived cell line was characterized, and its translation product, the E7 protein, was identified (D. Smotkin and F.O. Wettstein, Proc. Natl. Acad. Sci. USA 68:4680-4684, 1986). Here we show that the E7 protein had a half life of about 1 h and was located in the soluble cytoplasmic fraction. The protein was phosphorylated at serine residues and exhibited a high heterogeneous sedimentation rate in nondenaturing glycerol gradients, suggesting an oligomer formation or association with cellular protein.

Original languageEnglish (US)
Pages (from-to)1686-1689
Number of pages4
JournalJournal of Virology
Volume61
Issue number5
StatePublished - 1987
Externally publishedYes

Fingerprint

phosphoproteins
uterine cervical neoplasms
Papillomaviridae
Phosphoproteins
Uterine Cervical Neoplasms
Proteins
proteins
protein products
serine
translation (genetics)
half life
Human papillomavirus 16
glycerol
cell lines
Glycerol
Serine
Half-Life
neoplasms
Cell Line
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

The major human papillomavirus protein in cervical cancers is a cytoplasmic phosphoprotein. / Smotkin, David; Wettstein, F. O.

In: Journal of Virology, Vol. 61, No. 5, 1987, p. 1686-1689.

Research output: Contribution to journalArticle

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