The identification of monoclonal class switch variants by sib selection and an ELISA assay

Gad Spira, Antonio Bargellesi, Jean Luc Teillaud, Matthew D. Scharff

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Monoclonal antibodies can now be generated against a wide variety of antigens. However, a potentially useful monoclonal antibody may be of the wrong class or subclass for a particular task. Antibodies of the desired class can be obtained by identifying rare subclones in which the variable region has been rearranged to a new constant region. We describe here the use of sib selection and an enzyme-linked immunoassay (ELISA) for isolating such class and subclass switch variants from 2 IgM and 1 IgG3 producing hybridoma. The relative simplicity of ELISA assays make it feasible to apply this approach to many different hybridomas. Since commercial affinity purified class and subclass specific antibodies are now available, the method can be used by any laboratory. Furthermore, the technique does not require that the switch variants express surface immunoglobulin and enriches for hybridomas secreting higher amounts of antibody.

Original languageEnglish (US)
Pages (from-to)307-315
Number of pages9
JournalJournal of Immunological Methods
Volume74
Issue number2
DOIs
Publication statusPublished - Nov 30 1984

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Keywords

  • ELISA
  • cell line
  • class and subclass switch
  • monoclonal antibody
  • sib selection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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