The histone demethylase KDM5 is essential for larval growth in Drosophila

Coralie Drelon, Helen M. Belalcazar, Julie Secombe

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Regulated gene expression is necessary for developmental and homeostatic processes. The KDM5 family of transcriptional regulators are histone H3 lysine 4 demethylases that can function through both demethylase-dependent and-independent mechanisms. While loss and overexpression of KDM5 proteins are linked to intellectual disability and cancer, respectively, their normal developmental functions remain less characterized. Drosophila melanogaster provides an ideal system to investigate KDM5 function, as it encodes a single ortholog in contrast to the four paralogs found in mammalian cells. To examine the consequences of complete loss of KDM5, we generated a null allele of Drosophila kdm5, also known as little imaginal discs (lid), and show that it is essential for viability. Animals lacking KDM5 show a dramatically delayed larval development that coincides with decreased proliferation and increased cell death in wing imaginal discs. Interestingly, this developmental delay is independent of the well-characterized Jumonji C (JmjC) domain-encoded histone demethylase activity of KDM5, suggesting key functions for less characterized domains. Consistent with the phenotypes observed, transcriptome analyses of kdm5 null mutant wing imaginal discs revealed the dysregulation of genes involved in several cellular processes, including cell cycle progression and DNA repair. Together, our analyses reveal KDM5 as a key regulator of larval growth and offer an invaluable tool for defining the biological activities of KDM5 family proteins.

Original languageEnglish (US)
Pages (from-to)773-787
Number of pages15
JournalGenetics
Volume209
Issue number3
DOIs
StatePublished - Jul 1 2018

Fingerprint

Histone Demethylases
Imaginal Discs
Drosophila
Growth
Gene Expression Profiling
Drosophila melanogaster
Intellectual Disability
DNA Repair
Histones
Lysine
Cell Cycle
Proteins
Cell Death
Alleles
Phenotype
Gene Expression
Genes
Neoplasms

Keywords

  • Chromatin
  • H3K4me3
  • Imaginal disc
  • KDM5
  • Larval growth
  • Lid

ASJC Scopus subject areas

  • Genetics

Cite this

The histone demethylase KDM5 is essential for larval growth in Drosophila. / Drelon, Coralie; Belalcazar, Helen M.; Secombe, Julie.

In: Genetics, Vol. 209, No. 3, 01.07.2018, p. 773-787.

Research output: Contribution to journalArticle

Drelon, Coralie ; Belalcazar, Helen M. ; Secombe, Julie. / The histone demethylase KDM5 is essential for larval growth in Drosophila. In: Genetics. 2018 ; Vol. 209, No. 3. pp. 773-787.
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