The glucocorticoid receptor recognizes a specific nucleotide sequence in hepatitis B virus DNA causing increased activity of the HBV enhancer

Ran Tur-Kaspa, Yosef Shaul, David D. Moore, Robert D. Burk, Sam Okret, Lorenz Poellinger, David A. Shafritz

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The hepatitis B virus (HBV) genome contains a specific DNA binding site for the glucocorticoid receptor. Using DNaseI footprinting, this binding site was localized at HBV map positions 341-370 clockwise from the EcoRI site. The DNA sequence protected in the footprint contains two tandem copies of the GRE core hexanucleotide 5′-TGT CT-3′. Deletion analysis and reconstruction experiments in plasmid expression vectors demonstrated that this glucocorticoid receptor binding sequence serves as a signal for augmenting glucocorticoid-dependent activity of the HBV enhancer, which is located ∼ 730 nucleotides downstream in the HBV genome. Even though it does not serve as an independent enhancer element, the HBV glucocorticoid receptor domain can therefore be categorized as a functional GRE.

Original languageEnglish (US)
Pages (from-to)630-633
Number of pages4
JournalVirology
Volume167
Issue number2
StatePublished - Dec 1988

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Glucocorticoid Receptors
Hepatitis B virus
DNA
Binding Sites
Genome
Virus Receptors
Glucocorticoids
Plasmids
Nucleotides

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Tur-Kaspa, R., Shaul, Y., Moore, D. D., Burk, R. D., Okret, S., Poellinger, L., & Shafritz, D. A. (1988). The glucocorticoid receptor recognizes a specific nucleotide sequence in hepatitis B virus DNA causing increased activity of the HBV enhancer. Virology, 167(2), 630-633.

The glucocorticoid receptor recognizes a specific nucleotide sequence in hepatitis B virus DNA causing increased activity of the HBV enhancer. / Tur-Kaspa, Ran; Shaul, Yosef; Moore, David D.; Burk, Robert D.; Okret, Sam; Poellinger, Lorenz; Shafritz, David A.

In: Virology, Vol. 167, No. 2, 12.1988, p. 630-633.

Research output: Contribution to journalArticle

Tur-Kaspa, R, Shaul, Y, Moore, DD, Burk, RD, Okret, S, Poellinger, L & Shafritz, DA 1988, 'The glucocorticoid receptor recognizes a specific nucleotide sequence in hepatitis B virus DNA causing increased activity of the HBV enhancer', Virology, vol. 167, no. 2, pp. 630-633.
Tur-Kaspa, Ran ; Shaul, Yosef ; Moore, David D. ; Burk, Robert D. ; Okret, Sam ; Poellinger, Lorenz ; Shafritz, David A. / The glucocorticoid receptor recognizes a specific nucleotide sequence in hepatitis B virus DNA causing increased activity of the HBV enhancer. In: Virology. 1988 ; Vol. 167, No. 2. pp. 630-633.
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