The gene or not the gene-that is the question: Understanding the genetically engineered mouse phenotype

R. S. Sellers

Research output: Contribution to journalReview article

8 Scopus citations

Abstract

Embryonic stem cells have had a significant impact on understanding gene function and gene interactions through the use of genetically engineered mice. However, the genetic context (ie, mouse strain) in which these modifications in alleles are made may have a considerable effect on the phenotypic changes identified in these mice. In addition, tissue- and time-specific gene expression systems may generate unanticipated outcomes. This article discusses the history of embryonic stem cells, reviews how mouse strain can affect phenotype (using specific examples), and examines some of the caveats of conditional gene expression systems.

Original languageEnglish (US)
Pages (from-to)5-15
Number of pages11
JournalVeterinary Pathology
Volume49
Issue number1
DOIs
StatePublished - Jan 1 2012

Keywords

  • 129 strain
  • backcrossing
  • corpus callosum
  • embryonic carcinoma cells
  • embryonic stem cells
  • genetic background
  • modifier genes
  • phenotype
  • site-specific recombinase technology
  • tissue-specific gene expression

ASJC Scopus subject areas

  • veterinary(all)

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