The expression of IFN-γ, IL-4, Foxp3 and perforin genes are not correlated with DNA methylation status in patients with immune thrombocytopenic purpura

Haifeng Zhao, Weiting Du, Donghai Wang, Dongsheng Gu, Feng Xue, Jing Ge, Tao Sui, Renchi Yang

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

We investigated the relationship between the expression of IFN-γ, IL-4, Foxp3 and perforin gene and the methylation status of their promoters in immune thrombocytopenic purpura (ITP) patients. Gene expression and DNA methylation were determined by quantitative PCR and bisulfite genomic sequencing, respectively. The expression of IFN-γ was higher, while the expression of IL-4 was notably lower in ITP patients when compared with the controls. Most importantly, the Th1/Th2 (IFN-γ/IL-4) was remarkably higher in ITP patients, showing that the ITP patients were mainly in the Th1 polarization response. Although the methylation rate of CpGs located at -409 of perforin was higher (p=0.0376) and that of IFN-γ were relatively higher (p=0.0582) in ITP, we did not find the negative correlation between the expression of those genes and the methylation of their promoters.

Original languageEnglish (US)
Pages (from-to)137-143
Number of pages7
JournalPlatelets
Volume21
Issue number2
DOIs
Publication statusPublished - Feb 26 2010

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Keywords

  • DNA methylation
  • Foxp3
  • IFN-γ
  • IL-4
  • ITP
  • Perforin

ASJC Scopus subject areas

  • Hematology

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