The establishment of Caenorhabditis elegans germline pattern is controlled by overlapping proximal and distal somatic gonad signals

Anita S R Pepper, Te Wen Lo, Darrell J. Killian, David H. Hall, E. Jane Albert Hubbard

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We investigated the control of proliferation and differentiation in the larval Caenorhabditis elegans hermaphrodite germ line through analysis of glp-1 and lag-2 mutants, cell ablations, and ultrastructural data. After the first several rounds of germ cell division, GLP-1, a receptor of the LIN-12/Notch family, governs germline proliferation. We analyzed the proximal proliferation (Pro) phenotype in glp-1(ar202) and found that initial meiosis was delayed and spatially mispositioned. This is due, at least in part, to a heightened response of the mutant GLP-1 receptor to multiple sources of the somatic ligand LAG-2, including the proximal somatic gonad. We investigated whether proximal LAG-2 affects germline proliferation in the wild type. Our results indicate that (1) LAG-2 is necessary for GLP-1-mediated germline proliferation and prevention of early meiosis, and (2) several distinct anatomical sources of LAG-2 in the larval somatic gonad functionally overlap to promote proliferation and prevent early meiosis. Ultrastructural studies suggest that mitosis is not restricted to areas of direct DTC-germ line contact and that the germ line shares a common cytoplasm in larval stages. We propose that downregulation of the GLP-1 signaling pathway in the proximal germ line at the time of meiotic onset is under tight temporal and spatial control.

Original languageEnglish (US)
Pages (from-to)336-350
Number of pages15
JournalDevelopmental Biology
Volume259
Issue number2
DOIs
StatePublished - Jul 15 2003

Fingerprint

Caenorhabditis elegans
Gonads
Germ Cells
Meiosis
Glucagon-Like Peptide 1
Mitosis
Cell Division
Cytoplasm
Down-Regulation
Ligands
Phenotype
Glucagon-Like Peptide-1 Receptor

Keywords

  • C. elegans
  • Germ line
  • GLP-1/Notch
  • Gonadogenesis
  • LAG-2
  • Meiosis
  • Proliferation

ASJC Scopus subject areas

  • Developmental Biology

Cite this

The establishment of Caenorhabditis elegans germline pattern is controlled by overlapping proximal and distal somatic gonad signals. / Pepper, Anita S R; Lo, Te Wen; Killian, Darrell J.; Hall, David H.; Hubbard, E. Jane Albert.

In: Developmental Biology, Vol. 259, No. 2, 15.07.2003, p. 336-350.

Research output: Contribution to journalArticle

Pepper, Anita S R ; Lo, Te Wen ; Killian, Darrell J. ; Hall, David H. ; Hubbard, E. Jane Albert. / The establishment of Caenorhabditis elegans germline pattern is controlled by overlapping proximal and distal somatic gonad signals. In: Developmental Biology. 2003 ; Vol. 259, No. 2. pp. 336-350.
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