TY - JOUR
T1 - The Epstein-Barr virus origin of plasmid replication, oriP, contains both the initiation and termination sites of DNA replication
AU - Gahn, Toni A.
AU - Schildkraut, Carl L.
N1 - Funding Information:
We thank Dr. B. Sugden for helpful discussions. We also thank our laboratory colleagues Sabine Adolph, Veena Dhar, Kimi Hatton, and Bernardo Schvartzman for their suggestions and encouragement. The data in this paper are from a thesis to be submitted (T A. G.) in partial fulfillment of the requirements for the Degree of Doctor of Philosphy in the Sue Golding Graduate Division of Medical Sciences, Albert Einstein College of Medicine, Yeshiva University. These studies were supported by grants to C. L. S. from the American Cancer Society (CD-312) and the March of Dimes (I-1076). T A. G. was supported by a National Institutes of Health Training Grant (5 T32 CAOSOSO). Support was also provided by Cancer Core Grant (NIHINCI P3OCA13330).
PY - 1989/8/11
Y1 - 1989/8/11
N2 - Epstein-Barr virus (EBV) oriP contains two components, a dyad symmetry element and a direct repeat element, that, in the presence of EBV nuclear antigen 1, are necessary and sufficient for plasmid replication. We have examined the replicative forms generated by EBV oriP using 2D gel electrophoresis. The patterns obtained from an oriP plasmid in a transfected cell line indicate that the site of initiation of DNA replication is at or very near the dyad symmetry element, while the direct repeats contain a replication fork barrier and the termination site. Thus, replication from oriP proceeds in a predominantly unidirectional manner. The patterns obtained from cells immortalized by EBV suggest that replication from oriP proceeds similarly in the viral genome.
AB - Epstein-Barr virus (EBV) oriP contains two components, a dyad symmetry element and a direct repeat element, that, in the presence of EBV nuclear antigen 1, are necessary and sufficient for plasmid replication. We have examined the replicative forms generated by EBV oriP using 2D gel electrophoresis. The patterns obtained from an oriP plasmid in a transfected cell line indicate that the site of initiation of DNA replication is at or very near the dyad symmetry element, while the direct repeats contain a replication fork barrier and the termination site. Thus, replication from oriP proceeds in a predominantly unidirectional manner. The patterns obtained from cells immortalized by EBV suggest that replication from oriP proceeds similarly in the viral genome.
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U2 - 10.1016/0092-8674(89)90433-9
DO - 10.1016/0092-8674(89)90433-9
M3 - Article
C2 - 2547525
AN - SCOPUS:0024357214
VL - 58
SP - 527
EP - 535
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -