The effect of metheylmercury on glutamtne synthetase expression in cultured astrocytes

K. H. Tan, Michael Aschner

Research output: Contribution to journalArticle

Abstract

Glutaniine synthetase (GS) winch couverts glutamate and ammonia tu çhitaimti'1 plays essential roles in tin metabolism of neiirotra.nsmil.ters and the detoxification of ammonia in the brain. Tlio astrocyte-specific GS was found to he inhibited by very lov. concentrations of load salts and inorganic mercury, [his .itudy evaluated the effect on (IS expression in acntely-rnelhylmorcury I MeHg) exposed astrocyle culture?. A primary astrocyte culture was prepared from the cerebral cortex of new born rats and maintained for 21 days. The cells were then exposed to 10 u M Melig for fi hours or longer. Expression of G S in the control and MeHg <-xposed astrocytes were evaluated by western blotting ;md RT-PCR analyses. Total piotein was extracted from astrocyte.s and subjected to SDS-PAGF. and imirrino-detection. R.T-PCR was carried out using GS-specific primer pair and total RNA samples (0.25-1.0 ug) derived from the control and MeHg-treated astroe vtes. Results from both western blotting and HT Pt'R experiments demon-it rated significant increase in transcription of GS gene and translation of GS. I he increase in GS level in acutely-McHg exposes astrocytes presumably serve-; to aid the aMrocytes in maintaining glutamalc homeo.stasi.s in the brain. Nevertheless, the chronic effect of MeHg on rtstmrytrs ir, not clear (Supported by RIMI Nf'HK. NIH. 2 P20 R.R 1 1 58.1-02).

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997
Externally publishedYes

Fingerprint

astrocytes
Ligases
ligases
Astrocytes
Ammonia
Brain
Western blotting
ammonia
Western Blotting
reverse transcriptase polymerase chain reaction
winches
Winches
brain
Polymerase Chain Reaction
Detoxification
tin
Tin
cerebral cortex
Transcription
Mercury

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

The effect of metheylmercury on glutamtne synthetase expression in cultured astrocytes. / Tan, K. H.; Aschner, Michael.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

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