The Drosophila Shark tyrosine kinase is required for embryonic dorsal closure

Rafael Fernandez, Fumitaka Takahashi, Zhao Liu, Ruth Steward, David Stein, E. Richard Stanley

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Dorsal closure (DC) in the Drosophila embryo requires the coordinated interaction of two different functional domains of the epidermal cell layer - the leading edge (LE) and the lateral epidermis. In response to activation of a conserved c-Jun amino-terminal kinase (JNK) signaling module, the dorsal- most layer of cells, which constitute the LE of the stretching epithelial sheet, secrete Dpp, a member of the TGFβ superfamily. Dpp and other LE cell- derived signaling molecules stimulate the bilateral dorsal elongation of cells of the dorsolateral epidermis over the underlaying amnioserosa and the eventual fusion of their LEs along the dorsal midline. We have found that flies bearing a Shark tyrosine kinase gene mutation, shark1, exhibit a DC- defective phenotype. Dpp fails to be expressed in shark1 mutant LE cells. Consistent with these observations, epidermal-specific reconstitution of shark function or overexpression of an activated form of c-Jun in the shark1 mutant background, rescues the DC defect. Thus, Shark regulates the JNK signaling pathway leading to Dpp expression in LE cells. Furthermore, constitutive activation of the Dpp pathway throughout the epidermis fails to rescue the shark1 DC defect, suggesting that Shark may function in additional pathways in the LE and/or lateral epithelium.

Original languageEnglish (US)
Pages (from-to)604-614
Number of pages11
JournalGenes and Development
Volume14
Issue number5
StatePublished - Mar 1 2000

Fingerprint

Sharks
Epidermis
MAP Kinase Signaling System
JNK Mitogen-Activated Protein Kinases
Diptera
Protein-Tyrosine Kinases
Drosophila
Drosophila Shark protein
Embryonic Structures
Epithelium
Phenotype
Mutation
Genes

Keywords

  • Dorsal closure
  • Drosophila embryogenesis
  • Epithelial sheets
  • Jun kinase
  • Shark tyrosine kinase

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Fernandez, R., Takahashi, F., Liu, Z., Steward, R., Stein, D., & Stanley, E. R. (2000). The Drosophila Shark tyrosine kinase is required for embryonic dorsal closure. Genes and Development, 14(5), 604-614.

The Drosophila Shark tyrosine kinase is required for embryonic dorsal closure. / Fernandez, Rafael; Takahashi, Fumitaka; Liu, Zhao; Steward, Ruth; Stein, David; Stanley, E. Richard.

In: Genes and Development, Vol. 14, No. 5, 01.03.2000, p. 604-614.

Research output: Contribution to journalArticle

Fernandez, R, Takahashi, F, Liu, Z, Steward, R, Stein, D & Stanley, ER 2000, 'The Drosophila Shark tyrosine kinase is required for embryonic dorsal closure', Genes and Development, vol. 14, no. 5, pp. 604-614.
Fernandez R, Takahashi F, Liu Z, Steward R, Stein D, Stanley ER. The Drosophila Shark tyrosine kinase is required for embryonic dorsal closure. Genes and Development. 2000 Mar 1;14(5):604-614.
Fernandez, Rafael ; Takahashi, Fumitaka ; Liu, Zhao ; Steward, Ruth ; Stein, David ; Stanley, E. Richard. / The Drosophila Shark tyrosine kinase is required for embryonic dorsal closure. In: Genes and Development. 2000 ; Vol. 14, No. 5. pp. 604-614.
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