The DAM gene family encodes a new group of tumor-specific antigens recognized by human leukocyte antigen A2-restricted cytotoxic T lymphocytes

Katharina Fleischhauer, Luca Gattinoni, Piero Dalerba, Gregoire Lauvau, Elena Zanaria, Branca Dabovic, Peter M. Van Endert, Claudio Bordignon, Catia Traversari

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The DAM family of genes has a high degree of homology with MAGE, both in nucleotide sequence and in neoplastic tissue-specific expression. This study describes, for the first time, the identification of CTLs specific for a peptide epitope encoded by DAM genes. A human leukocyte antigen (HLA)-A2- restricted CTL clone was raised against a peptide, D10/6-271, encoded by codons 271-279 in the DAM cDNA. The corresponding peptide in the MAGE-3 sequence, M3-271, has been shown previously to be a natural T-cell epitope for HLA-A2-restricted CTLs recognizing the MAGE-3 protein. The D10/6-271- specific CTL clone required approximately 3 nM exogenous peptide for half- maximal lysis of target cells and was able to specifically recognize endogenous DAM antigen on HLA-A2+ melanoma cells infected with a vaccinia vector recombinant for gene DAM-6. These data suggest that DAM genes might encode a new group of tumor-specific antigens useful for the design of specific antitumor vaccines.

Original languageEnglish (US)
Pages (from-to)2969-2972
Number of pages4
JournalCancer Research
Volume58
Issue number14
StatePublished - Jul 15 1998
Externally publishedYes

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Cytotoxic T-Lymphocytes
Neoplasm Antigens
HLA Antigens
varespladib methyl
Peptides
Genes
Clone Cells
Vaccinia
T-Lymphocyte Epitopes
Codon
Epitopes
Melanoma
Vaccines
Complementary DNA
Antigens
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Fleischhauer, K., Gattinoni, L., Dalerba, P., Lauvau, G., Zanaria, E., Dabovic, B., ... Traversari, C. (1998). The DAM gene family encodes a new group of tumor-specific antigens recognized by human leukocyte antigen A2-restricted cytotoxic T lymphocytes. Cancer Research, 58(14), 2969-2972.

The DAM gene family encodes a new group of tumor-specific antigens recognized by human leukocyte antigen A2-restricted cytotoxic T lymphocytes. / Fleischhauer, Katharina; Gattinoni, Luca; Dalerba, Piero; Lauvau, Gregoire; Zanaria, Elena; Dabovic, Branca; Van Endert, Peter M.; Bordignon, Claudio; Traversari, Catia.

In: Cancer Research, Vol. 58, No. 14, 15.07.1998, p. 2969-2972.

Research output: Contribution to journalArticle

Fleischhauer, K, Gattinoni, L, Dalerba, P, Lauvau, G, Zanaria, E, Dabovic, B, Van Endert, PM, Bordignon, C & Traversari, C 1998, 'The DAM gene family encodes a new group of tumor-specific antigens recognized by human leukocyte antigen A2-restricted cytotoxic T lymphocytes', Cancer Research, vol. 58, no. 14, pp. 2969-2972.
Fleischhauer, Katharina ; Gattinoni, Luca ; Dalerba, Piero ; Lauvau, Gregoire ; Zanaria, Elena ; Dabovic, Branca ; Van Endert, Peter M. ; Bordignon, Claudio ; Traversari, Catia. / The DAM gene family encodes a new group of tumor-specific antigens recognized by human leukocyte antigen A2-restricted cytotoxic T lymphocytes. In: Cancer Research. 1998 ; Vol. 58, No. 14. pp. 2969-2972.
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