The DAM gene family encodes a new group of tumor-specific antigens recognized by human leukocyte antigen A2-restricted cytotoxic T lymphocytes

Katharina Fleischhauer, Luca Gattinoni, Piero Dalerba, Grégoire Lauvau, Elena Zanaria, Branca Dabovic, Peter M. Van Endert, Claudio Bordignon, Catia Traversari

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Abstract

The DAM family of genes has a high degree of homology with MAGE, both in nucleotide sequence and in neoplastic tissue-specific expression. This study describes, for the first time, the identification of CTLs specific for a peptide epitope encoded by DAM genes. A human leukocyte antigen (HLA)-A2- restricted CTL clone was raised against a peptide, D10/6-271, encoded by codons 271-279 in the DAM cDNA. The corresponding peptide in the MAGE-3 sequence, M3-271, has been shown previously to be a natural T-cell epitope for HLA-A2-restricted CTLs recognizing the MAGE-3 protein. The D10/6-271- specific CTL clone required approximately 3 nM exogenous peptide for half- maximal lysis of target cells and was able to specifically recognize endogenous DAM antigen on HLA-A2+ melanoma cells infected with a vaccinia vector recombinant for gene DAM-6. These data suggest that DAM genes might encode a new group of tumor-specific antigens useful for the design of specific antitumor vaccines.

Original languageEnglish (US)
Pages (from-to)2969-2972
Number of pages4
JournalCancer Research
Volume58
Issue number14
Publication statusPublished - Jul 15 1998
Externally publishedYes

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Fleischhauer, K., Gattinoni, L., Dalerba, P., Lauvau, G., Zanaria, E., Dabovic, B., ... Traversari, C. (1998). The DAM gene family encodes a new group of tumor-specific antigens recognized by human leukocyte antigen A2-restricted cytotoxic T lymphocytes. Cancer Research, 58(14), 2969-2972.