The cloning of a receptor-type protein tyrosine phosphatase expressed in the central nervous system

J. B. Levy, P. D. Canoll, O. Silvennoinen, G. Barnea, B. Morse, A. M. Honeggerr, J. T. Huang, L. A. Cannizzaro, S. H. Park, T. Druck, K. Huebner, J. Sap, M. Ehrlich, J. M. Musacchio, J. Schlessinger

Research output: Contribution to journalArticle

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Abstract

We have isolated cDNA clones and deduced the complete amino acid sequence of a large receptor-type protein tyrosine phosphatase containing 2307 amino acids. The human gene encoding this phosphatase, denoted RPTPβ (or PTPζ), has been localized to chromosome 7q31-33. RPTPβ is composed of a large extracellular domain, a single transmembrane domain, and a cytoplasmic portion with two tandem catalytic domains. We have also cloned a variant of RPTPβ lacking 859 amino acids from the extracellular domain but with intact transmembrane and cytoplasmic domains. Interestingly, the amino-terminal region of the extracellular domain of RPTPβ contains a stretch of 266 amino acids with striking homology to the enzyme carbonic anhydrase. Immunoprecipitation experiments from a human neuroblastoma cell line indicate that the apparent molecular mass of the core and glycosylated forms of RPTPβ are approximately 250 and 300 kDa, respectively. Northern blot analysis shows that RPTPβ is strictly expressed in the central nervous system. In situ hybridization was used to further localize the expression to different regions of the adult brain including the Purkinje cell layer of the cerebellum, the dentate gyrus, and the subependymal layer of the anterior horn of the lateral ventricle. Hence, RPTPβ represents the first mammalian tyrosine phosphatase whose expression is restricted to the nervous system. The high level of expression of RPTPβ transcripts in the ventricular and subventricular zones of the embryonic mouse brain suggests the importance of this tyrosine phosphatase in the development of the central nervous system.

Original languageEnglish (US)
Pages (from-to)10573-10581
Number of pages9
JournalJournal of Biological Chemistry
Volume268
Issue number14
StatePublished - May 15 1993
Externally publishedYes

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Protein Tyrosine Phosphatases
Cloning
Neurology
Phosphoric Monoester Hydrolases
Organism Cloning
Central Nervous System
Lateral Ventricles
Amino Acids
Tyrosine
Brain
Carbonic Anhydrases
Purkinje Cells
Cells
Dentate Gyrus
Horns
Neuroblastoma
Immunoprecipitation
Northern Blotting
Cerebellum
Nervous System

ASJC Scopus subject areas

  • Biochemistry

Cite this

Levy, J. B., Canoll, P. D., Silvennoinen, O., Barnea, G., Morse, B., Honeggerr, A. M., ... Schlessinger, J. (1993). The cloning of a receptor-type protein tyrosine phosphatase expressed in the central nervous system. Journal of Biological Chemistry, 268(14), 10573-10581.

The cloning of a receptor-type protein tyrosine phosphatase expressed in the central nervous system. / Levy, J. B.; Canoll, P. D.; Silvennoinen, O.; Barnea, G.; Morse, B.; Honeggerr, A. M.; Huang, J. T.; Cannizzaro, L. A.; Park, S. H.; Druck, T.; Huebner, K.; Sap, J.; Ehrlich, M.; Musacchio, J. M.; Schlessinger, J.

In: Journal of Biological Chemistry, Vol. 268, No. 14, 15.05.1993, p. 10573-10581.

Research output: Contribution to journalArticle

Levy, JB, Canoll, PD, Silvennoinen, O, Barnea, G, Morse, B, Honeggerr, AM, Huang, JT, Cannizzaro, LA, Park, SH, Druck, T, Huebner, K, Sap, J, Ehrlich, M, Musacchio, JM & Schlessinger, J 1993, 'The cloning of a receptor-type protein tyrosine phosphatase expressed in the central nervous system', Journal of Biological Chemistry, vol. 268, no. 14, pp. 10573-10581.
Levy JB, Canoll PD, Silvennoinen O, Barnea G, Morse B, Honeggerr AM et al. The cloning of a receptor-type protein tyrosine phosphatase expressed in the central nervous system. Journal of Biological Chemistry. 1993 May 15;268(14):10573-10581.
Levy, J. B. ; Canoll, P. D. ; Silvennoinen, O. ; Barnea, G. ; Morse, B. ; Honeggerr, A. M. ; Huang, J. T. ; Cannizzaro, L. A. ; Park, S. H. ; Druck, T. ; Huebner, K. ; Sap, J. ; Ehrlich, M. ; Musacchio, J. M. ; Schlessinger, J. / The cloning of a receptor-type protein tyrosine phosphatase expressed in the central nervous system. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 14. pp. 10573-10581.
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AU - Morse, B.

AU - Honeggerr, A. M.

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AU - Huebner, K.

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