The choroid plexus epithelium is the site of the organic anion transport protein in the brain

Ruth Hogue Angeletti, Phyllis M. Novikoff, Shailaja Rao Juvvadi, Jean Marc Fritschy, Peter J. Meier, Allan W. Wolkoff

Research output: Contribution to journalArticlepeer-review

124 Scopus citations

Abstract

The mRNA for organic anion transport protein (oatp) was previously shown to be present in abundance in liver and kidney, and in small amounts in brain. Data obtained from experiments with reverse transcriptase-PCR techniques and in situ hybridization analysis showed that the oatp mRNA is present within the brain, localized to the choroid plexus. A sequence- specific antibody to the oatp polypeptide demonstrated the presence of the expected polypeptide with a molecular weight of 80,000 plus an immunoreactive species with a higher molecular weight in preparations of choroid plexus membranes. Examination of the choroid plexus by fluorescence confocal microscopy revealed that immunoreactive oatp polypeptide is localized to the apical surface of the choroid plexus epithelial cells, which contacts the cerebrospinal fluid. This localization of oatp is consistent with previous experiments showing vectorial transport of organic anions between the choroid plexus and the cerebrospinal fluid.

Original languageEnglish (US)
Pages (from-to)283-286
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number1
DOIs
StatePublished - Jan 7 1997

Keywords

  • bilirubin
  • oatp

ASJC Scopus subject areas

  • General

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