The chemokine interleukin-8 regulates parathyroid secretion

Ruth Hogue Angeletti, Thomas D'Amico, Sheela Ashok, John Russell

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Interleukin-8 (IL-8) is a chemokine important in inflammatory processes. Homology cloning experiments performed using bovine parathyroid cDNA and degenerate primers encoding transmembrane regions III and VI of peptide and protein hormone G-protein coupled receptors identified a set of known receptors not previously identified in the parathyroid. Among these was the IL-8 type B receptor. Incubation of freshly isolated bovine parathyroid cells with recombinant IL-8 for 6-48 h produced an increase in the levels of mRNA for parathyroid hormone (PTH). The levels of PTH secreted in response to nanomolar amounts of IL-8 were also elevated in cells incubated for 1 h with IL-8. Differential display analysis of mRNA from parathyroid cells, incubated in the presence and absence of IL-8, permitted the identification of cDNA clones for RNA species whose expression was either elevated or suppressed. These experiments suggest that IL-8 and inflammatory events play a role in bone homeostasis through actions on the parathyroid gland.

Original languageEnglish (US)
Pages (from-to)1232-1237
Number of pages6
JournalJournal of Bone and Mineral Research
Volume13
Issue number8
DOIs
StatePublished - Aug 1998

Fingerprint

Interleukin-8
Chemokines
Parathyroid Hormone
Interleukin-8B Receptors
Complementary DNA
Parathyroid Glands
Peptide Hormones
Gene Expression Profiling
G-Protein-Coupled Receptors
Organism Cloning
Interleukin-6
Homeostasis
Clone Cells
RNA
Bone and Bones
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Surgery

Cite this

The chemokine interleukin-8 regulates parathyroid secretion. / Angeletti, Ruth Hogue; D'Amico, Thomas; Ashok, Sheela; Russell, John.

In: Journal of Bone and Mineral Research, Vol. 13, No. 8, 08.1998, p. 1232-1237.

Research output: Contribution to journalArticle

Angeletti, RH, D'Amico, T, Ashok, S & Russell, J 1998, 'The chemokine interleukin-8 regulates parathyroid secretion', Journal of Bone and Mineral Research, vol. 13, no. 8, pp. 1232-1237. https://doi.org/10.1359/jbmr.1998.13.8.1232
Angeletti, Ruth Hogue ; D'Amico, Thomas ; Ashok, Sheela ; Russell, John. / The chemokine interleukin-8 regulates parathyroid secretion. In: Journal of Bone and Mineral Research. 1998 ; Vol. 13, No. 8. pp. 1232-1237.
@article{09b1e4e5af1547ac82669ee2fc574bb9,
title = "The chemokine interleukin-8 regulates parathyroid secretion",
abstract = "Interleukin-8 (IL-8) is a chemokine important in inflammatory processes. Homology cloning experiments performed using bovine parathyroid cDNA and degenerate primers encoding transmembrane regions III and VI of peptide and protein hormone G-protein coupled receptors identified a set of known receptors not previously identified in the parathyroid. Among these was the IL-8 type B receptor. Incubation of freshly isolated bovine parathyroid cells with recombinant IL-8 for 6-48 h produced an increase in the levels of mRNA for parathyroid hormone (PTH). The levels of PTH secreted in response to nanomolar amounts of IL-8 were also elevated in cells incubated for 1 h with IL-8. Differential display analysis of mRNA from parathyroid cells, incubated in the presence and absence of IL-8, permitted the identification of cDNA clones for RNA species whose expression was either elevated or suppressed. These experiments suggest that IL-8 and inflammatory events play a role in bone homeostasis through actions on the parathyroid gland.",
author = "Angeletti, {Ruth Hogue} and Thomas D'Amico and Sheela Ashok and John Russell",
year = "1998",
month = "8",
doi = "10.1359/jbmr.1998.13.8.1232",
language = "English (US)",
volume = "13",
pages = "1232--1237",
journal = "Journal of Bone and Mineral Research",
issn = "0884-0431",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - The chemokine interleukin-8 regulates parathyroid secretion

AU - Angeletti, Ruth Hogue

AU - D'Amico, Thomas

AU - Ashok, Sheela

AU - Russell, John

PY - 1998/8

Y1 - 1998/8

N2 - Interleukin-8 (IL-8) is a chemokine important in inflammatory processes. Homology cloning experiments performed using bovine parathyroid cDNA and degenerate primers encoding transmembrane regions III and VI of peptide and protein hormone G-protein coupled receptors identified a set of known receptors not previously identified in the parathyroid. Among these was the IL-8 type B receptor. Incubation of freshly isolated bovine parathyroid cells with recombinant IL-8 for 6-48 h produced an increase in the levels of mRNA for parathyroid hormone (PTH). The levels of PTH secreted in response to nanomolar amounts of IL-8 were also elevated in cells incubated for 1 h with IL-8. Differential display analysis of mRNA from parathyroid cells, incubated in the presence and absence of IL-8, permitted the identification of cDNA clones for RNA species whose expression was either elevated or suppressed. These experiments suggest that IL-8 and inflammatory events play a role in bone homeostasis through actions on the parathyroid gland.

AB - Interleukin-8 (IL-8) is a chemokine important in inflammatory processes. Homology cloning experiments performed using bovine parathyroid cDNA and degenerate primers encoding transmembrane regions III and VI of peptide and protein hormone G-protein coupled receptors identified a set of known receptors not previously identified in the parathyroid. Among these was the IL-8 type B receptor. Incubation of freshly isolated bovine parathyroid cells with recombinant IL-8 for 6-48 h produced an increase in the levels of mRNA for parathyroid hormone (PTH). The levels of PTH secreted in response to nanomolar amounts of IL-8 were also elevated in cells incubated for 1 h with IL-8. Differential display analysis of mRNA from parathyroid cells, incubated in the presence and absence of IL-8, permitted the identification of cDNA clones for RNA species whose expression was either elevated or suppressed. These experiments suggest that IL-8 and inflammatory events play a role in bone homeostasis through actions on the parathyroid gland.

UR - http://www.scopus.com/inward/record.url?scp=0031828429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031828429&partnerID=8YFLogxK

U2 - 10.1359/jbmr.1998.13.8.1232

DO - 10.1359/jbmr.1998.13.8.1232

M3 - Article

VL - 13

SP - 1232

EP - 1237

JO - Journal of Bone and Mineral Research

JF - Journal of Bone and Mineral Research

SN - 0884-0431

IS - 8

ER -