The C. elegans Polycomb gene sop-2 encodes an RNA binding protein

Hong Zhang, Andrea Christoforou, L. Aravind, Scott W. Emmons, Sander Van Den Heuvel, Daniel A. Haber

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Epigenetic silencing of Hox cluster genes by Polycomb group (PcG) proteins is thought to involve the formation of a stably inherited repressive chromatin structure. Here we show that the C. elegans-specific PcG protein SOP-2 directly binds to RNA through three nonoverlapping regions, each of which is essential for its localization to characteristic nuclear bodies and for its in vivo function in the repression of Hox genes. Functional studies indicate that the RNA involved in SOP-2 binding is distinct from either siRNA or microRNA. Remarkably, the vertebrate PcG protein Rae28, which is functionally and structurally related to SOP-2, also binds to RNA through an FCS finger domain. Substitution of the Rae28 FCS finger for the essential RNA binding region of SOP-2 partially restores localization to nuclear bodies. These observations suggest that direct binding to RNA is an evolutionarily conserved and potentially important property of PcG proteins.

Original languageEnglish (US)
Pages (from-to)841-847
Number of pages7
JournalMolecular Cell
Volume14
Issue number6
DOIs
StatePublished - Jun 18 2004

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RNA-Binding Proteins
Polycomb-Group Proteins
RNA
Homeobox Genes
Genes
Fingers
MicroRNAs
Epigenomics
Small Interfering RNA
Chromatin
Vertebrates

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Zhang, H., Christoforou, A., Aravind, L., Emmons, S. W., Van Den Heuvel, S., & Haber, D. A. (2004). The C. elegans Polycomb gene sop-2 encodes an RNA binding protein. Molecular Cell, 14(6), 841-847. https://doi.org/10.1016/j.molcel.2004.06.001

The C. elegans Polycomb gene sop-2 encodes an RNA binding protein. / Zhang, Hong; Christoforou, Andrea; Aravind, L.; Emmons, Scott W.; Van Den Heuvel, Sander; Haber, Daniel A.

In: Molecular Cell, Vol. 14, No. 6, 18.06.2004, p. 841-847.

Research output: Contribution to journalArticle

Zhang, H, Christoforou, A, Aravind, L, Emmons, SW, Van Den Heuvel, S & Haber, DA 2004, 'The C. elegans Polycomb gene sop-2 encodes an RNA binding protein', Molecular Cell, vol. 14, no. 6, pp. 841-847. https://doi.org/10.1016/j.molcel.2004.06.001
Zhang H, Christoforou A, Aravind L, Emmons SW, Van Den Heuvel S, Haber DA. The C. elegans Polycomb gene sop-2 encodes an RNA binding protein. Molecular Cell. 2004 Jun 18;14(6):841-847. https://doi.org/10.1016/j.molcel.2004.06.001
Zhang, Hong ; Christoforou, Andrea ; Aravind, L. ; Emmons, Scott W. ; Van Den Heuvel, Sander ; Haber, Daniel A. / The C. elegans Polycomb gene sop-2 encodes an RNA binding protein. In: Molecular Cell. 2004 ; Vol. 14, No. 6. pp. 841-847.
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