The Application of Molecular Diagnostics to Stained Cytology Smears

Maja H. Oktay, Esther Adler, Laleh Hakima, Eli Grunblatt, Evan Pieri, Andrew Seymour, Samer N. Khader, Antonio Cajigas, Mark J. Suhrland, Sumanta Goswami

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Detection of mutational alterations is important for guiding treatment decisions of lung non-small-cell carcinomas and thyroid nodules with atypical cytologic findings. Inoperable lung tumors requiring further testing for staging and thyroid lesions often are diagnosed using only cytology material. Molecular diagnostic tests of these samples typically are performed on cell blocks; however, insufficient cellularity of cell blocks is a limitation for test performance. In addition, some of the fixatives used while preparing cell blocks often introduces artifacts for mutation detection. Here, we applied qClamp xenonucleic technology and quantitative RT-PCR to cells microdissected directly from stained cytology smears to detect common alterations including mutations and translocations in non-small-cell carcinomas and thyroid lesions. By using this approach, we achieved a 1% molecular alteration detection rate from as few as 50 cells. Ultrasensitive methods of molecular alteration detection similar to the one described here will be increasingly important for the evaluation of molecular alterations in clinical scenarios when only tissue samples that are small are available.

Original languageEnglish (US)
Pages (from-to)407-415
Number of pages9
JournalJournal of Molecular Diagnostics
Volume18
Issue number3
DOIs
StatePublished - May 1 2016

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Molecular Pathology
Cell Biology
Fixatives
Mutation
Thyroid Nodule
Thyroid Neoplasms
Routine Diagnostic Tests
Non-Small Cell Lung Carcinoma
Artifacts
Thyroid Gland
Technology
Polymerase Chain Reaction
Lung
Neoplasms

ASJC Scopus subject areas

  • Molecular Medicine
  • Pathology and Forensic Medicine

Cite this

The Application of Molecular Diagnostics to Stained Cytology Smears. / Oktay, Maja H.; Adler, Esther; Hakima, Laleh; Grunblatt, Eli; Pieri, Evan; Seymour, Andrew; Khader, Samer N.; Cajigas, Antonio; Suhrland, Mark J.; Goswami, Sumanta.

In: Journal of Molecular Diagnostics, Vol. 18, No. 3, 01.05.2016, p. 407-415.

Research output: Contribution to journalArticle

Oktay, Maja H. ; Adler, Esther ; Hakima, Laleh ; Grunblatt, Eli ; Pieri, Evan ; Seymour, Andrew ; Khader, Samer N. ; Cajigas, Antonio ; Suhrland, Mark J. ; Goswami, Sumanta. / The Application of Molecular Diagnostics to Stained Cytology Smears. In: Journal of Molecular Diagnostics. 2016 ; Vol. 18, No. 3. pp. 407-415.
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