The alternatively-included 11a sequence modifies the effects of Mena on actin cytoskeletal organization and cell behavior

Michele Balsamo, Chandrani Mondal, Guillaume Carmona, Leslie M. McClain, Daisy N. Riquelme, Jenny Tadros, Duan Ma, Eliza Vasile, John S. Condeelis, Douglas A. Lauffenburger, Frank B. Gertler

Research output: Contribution to journalArticle

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Abstract

During tumor progression, alternative splicing gives rise to different Mena protein isoforms. We analyzed how Mena11a, an isoform enriched in epithelia and epithelial-like cells, affects Mena-dependent regulation of actin dynamics and cell behavior. While other Mena isoforms promote actin polymerization and drive membrane protrusion, we find that Mena11a decreases actin polymerization and growth factor-stimulated membrane protrusion at lamellipodia. Ectopic Mena11a expression slows mesenchymal-like cell motility, while isoform-specific depletion of endogenous Mena11a in epithelial-like tumor cells perturbs cell:cell junctions and increases membrane protrusion and overall cell motility. Mena11a can dampen membrane protrusion and reduce actin polymerization in the absence of other Mena isoforms, indicating that it is not simply an inactive Mena isoform. We identify a phosphorylation site within 11a that is required for some Mena11a-specific functions. RNA-seq data analysis from patient cohorts demonstrates that the difference between mRNAs encoding constitutive Mena sequences and those containing the 11a exon correlates with metastasis in colorectal cancer, suggesting that 11a exon exclusion contributes to invasive phenotypes and leads to poor clinical outcomes.

Original languageEnglish (US)
Article number35298
JournalScientific Reports
Volume6
DOIs
StatePublished - Oct 17 2016

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Actins
Protein Isoforms
Polymerization
Membranes
Cell Movement
Exons
Pseudopodia
Intercellular Junctions
Alternative Splicing
Colorectal Neoplasms
Neoplasms
Intercellular Signaling Peptides and Proteins
Epithelium
Epithelial Cells
Phosphorylation
RNA
Neoplasm Metastasis
Phenotype
Messenger RNA

ASJC Scopus subject areas

  • General

Cite this

Balsamo, M., Mondal, C., Carmona, G., McClain, L. M., Riquelme, D. N., Tadros, J., ... Gertler, F. B. (2016). The alternatively-included 11a sequence modifies the effects of Mena on actin cytoskeletal organization and cell behavior. Scientific Reports, 6, [35298]. https://doi.org/10.1038/srep35298

The alternatively-included 11a sequence modifies the effects of Mena on actin cytoskeletal organization and cell behavior. / Balsamo, Michele; Mondal, Chandrani; Carmona, Guillaume; McClain, Leslie M.; Riquelme, Daisy N.; Tadros, Jenny; Ma, Duan; Vasile, Eliza; Condeelis, John S.; Lauffenburger, Douglas A.; Gertler, Frank B.

In: Scientific Reports, Vol. 6, 35298, 17.10.2016.

Research output: Contribution to journalArticle

Balsamo, M, Mondal, C, Carmona, G, McClain, LM, Riquelme, DN, Tadros, J, Ma, D, Vasile, E, Condeelis, JS, Lauffenburger, DA & Gertler, FB 2016, 'The alternatively-included 11a sequence modifies the effects of Mena on actin cytoskeletal organization and cell behavior', Scientific Reports, vol. 6, 35298. https://doi.org/10.1038/srep35298
Balsamo, Michele ; Mondal, Chandrani ; Carmona, Guillaume ; McClain, Leslie M. ; Riquelme, Daisy N. ; Tadros, Jenny ; Ma, Duan ; Vasile, Eliza ; Condeelis, John S. ; Lauffenburger, Douglas A. ; Gertler, Frank B. / The alternatively-included 11a sequence modifies the effects of Mena on actin cytoskeletal organization and cell behavior. In: Scientific Reports. 2016 ; Vol. 6.
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