The AFF-1 exoplasmic fusogen is required for endocytic scission and seamless tube elongation

Fabien Soulavie, David H. Hall, Meera V. Sundaram

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Many membranes must merge during cellular trafficking, but fusion and fission events initiating at exoplasmic (non-cytosolic) membrane surfaces are not well understood. Here we show that the C. elegans cell-cell fusogen anchor-cell fusion failure 1 (AFF-1) is required for membrane trafficking events during development of a seamless unicellular tube. EGF-Ras-ERK signaling upregulates AFF-1 expression in the excretory duct tube to promote tube auto-fusion and subsequent lumen elongation. AFF-1 is required for scission of basal endocytic compartments and for apically directed exocytosis to extend the apical membrane. Lumen elongation also requires the transcytosis factor Rab11, but occurs independently of dynamin and clathrin. These results support a transcytosis model of seamless tube lumen growth and show that cell-cell fusogens also can play roles in intracellular membrane trafficking events.

Original languageEnglish (US)
Article number1741
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

Cell Fusion
Anchors
elongation
Elongation
cleavage
Fusion reactions
fusion
tubes
Membranes
Transcytosis
lumens
cells
membranes
Dynamins
Clathrin
Intracellular Membranes
Exocytosis
Epidermal Growth Factor
Up-Regulation
Ducts

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

The AFF-1 exoplasmic fusogen is required for endocytic scission and seamless tube elongation. / Soulavie, Fabien; Hall, David H.; Sundaram, Meera V.

In: Nature Communications, Vol. 9, No. 1, 1741, 01.12.2018.

Research output: Contribution to journalArticle

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