@article{f867adb88d5f4e4b8b2883e7aceda462,
title = "The AFF-1 exoplasmic fusogen is required for endocytic scission and seamless tube elongation",
abstract = "Many membranes must merge during cellular trafficking, but fusion and fission events initiating at exoplasmic (non-cytosolic) membrane surfaces are not well understood. Here we show that the C. elegans cell-cell fusogen anchor-cell fusion failure 1 (AFF-1) is required for membrane trafficking events during development of a seamless unicellular tube. EGF-Ras-ERK signaling upregulates AFF-1 expression in the excretory duct tube to promote tube auto-fusion and subsequent lumen elongation. AFF-1 is required for scission of basal endocytic compartments and for apically directed exocytosis to extend the apical membrane. Lumen elongation also requires the transcytosis factor Rab11, but occurs independently of dynamin and clathrin. These results support a transcytosis model of seamless tube lumen growth and show that cell-cell fusogens also can play roles in intracellular membrane trafficking events.",
author = "Fabien Soulavie and Hall, {David H.} and Sundaram, {Meera V.}",
note = "Funding Information: We thank Ken Nguyen, Leslie Gunther-Cummins, and Geoff Perumal for help with electron microscopy, Benjamin Podbilewicz and Max Heiman for providing reagents, Benjamin Podbilewicz, Barth Grant, Chris Rocheleau, Sergio Grinstein, Bob Doms, Mickey Marks, and members of the UPenn C. elegans community for helpful discussions and advice, Jennifer Cohen for artwork, Rachel Forman-Rubinsky for technical assistance, and Piya Ghose and Shai Shaham for sharing unpublished data. We thank Jonathan Hodgkin for help in transferring the files of Nichol Thomson (MRC/LMB) to the Hall lab, for sharing on www.wormimage.org. Some strains were provided by the Caenorhabiditis Genetics Center (CGC), which is funded by the NIH Office of Research Infrastructure Programs (P40 OD01440). This work was funded by National Institutes of Health grants R01GM58540 to M.V.S. and OD010943 to D.H.H. (with permission). Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = dec,
day = "1",
doi = "10.1038/s41467-018-04091-1",
language = "English (US)",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}