Taxol-induced Bcl-2 phosphorylation in ovarian cancer cell monolayer and spheroids

S. Makhija; D. D. Taylor; R. K. Gibb; Ç Gerçel-Taylor

doi:10.3892/ijo.14.3.515

Taxol-induced Bcl-2 phosphorylation in ovarian cancer cell monolayer and spheroids

S. Makhija, D. D. Taylor, R. K. Gibb, Ç Gerçel-Taylor

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Expression of Bcl-2, Bax, p53 and induction of apoptosis were studied in cisplatin or Taxol treated monolayer and spheroid cultures of ovarian cancer cell lines (SKOV-3, UL-1, UL-3C). While cisplatin (15-75 μg/ml) induced apoptosis in monolayer and spheroid cultures, Taxol (100-800 nM) induced fragmentation in monolayers only. Cisplatin induced up to 5-fold DNA fragmentation in monolayers, while 3-fold (UL-3C, SKOV-3), and 1.5-fold (UL-1) in spheroids. Taxol treatment of monolayers resulted in the characteristic phosphorylation of Bcl-2, which was not demonstrated in spheroid cultures. Bax expression was reduced in spheroids following cisplatin or Taxol treatment, while p53 levels remained unchanged.

Original language	English (US)
Pages (from-to)	515-521
Number of pages	7
Journal	International journal of oncology
Volume	14
Issue number	3
DOIs	https://doi.org/10.3892/ijo.14.3.515
State	Published - Mar 1999
Externally published	Yes

Keywords

Bcl-2
Ovarian cancer
Phosphorylation
Spheroids
Taxol

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3892/ijo.14.3.515

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title = "Taxol-induced Bcl-2 phosphorylation in ovarian cancer cell monolayer and spheroids",

abstract = "Expression of Bcl-2, Bax, p53 and induction of apoptosis were studied in cisplatin or Taxol treated monolayer and spheroid cultures of ovarian cancer cell lines (SKOV-3, UL-1, UL-3C). While cisplatin (15-75 μg/ml) induced apoptosis in monolayer and spheroid cultures, Taxol (100-800 nM) induced fragmentation in monolayers only. Cisplatin induced up to 5-fold DNA fragmentation in monolayers, while 3-fold (UL-3C, SKOV-3), and 1.5-fold (UL-1) in spheroids. Taxol treatment of monolayers resulted in the characteristic phosphorylation of Bcl-2, which was not demonstrated in spheroid cultures. Bax expression was reduced in spheroids following cisplatin or Taxol treatment, while p53 levels remained unchanged.",

keywords = "Bcl-2, Ovarian cancer, Phosphorylation, Spheroids, Taxol",

author = "S. Makhija and Taylor, {D. D.} and Gibb, {R. K.} and {\c C} Ger{\c c}el-Taylor",

year = "1999",

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doi = "10.3892/ijo.14.3.515",

language = "English (US)",

volume = "14",

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T1 - Taxol-induced Bcl-2 phosphorylation in ovarian cancer cell monolayer and spheroids

AU - Makhija, S.

AU - Taylor, D. D.

AU - Gibb, R. K.

AU - Gerçel-Taylor, Ç

PY - 1999/3

Y1 - 1999/3

N2 - Expression of Bcl-2, Bax, p53 and induction of apoptosis were studied in cisplatin or Taxol treated monolayer and spheroid cultures of ovarian cancer cell lines (SKOV-3, UL-1, UL-3C). While cisplatin (15-75 μg/ml) induced apoptosis in monolayer and spheroid cultures, Taxol (100-800 nM) induced fragmentation in monolayers only. Cisplatin induced up to 5-fold DNA fragmentation in monolayers, while 3-fold (UL-3C, SKOV-3), and 1.5-fold (UL-1) in spheroids. Taxol treatment of monolayers resulted in the characteristic phosphorylation of Bcl-2, which was not demonstrated in spheroid cultures. Bax expression was reduced in spheroids following cisplatin or Taxol treatment, while p53 levels remained unchanged.

AB - Expression of Bcl-2, Bax, p53 and induction of apoptosis were studied in cisplatin or Taxol treated monolayer and spheroid cultures of ovarian cancer cell lines (SKOV-3, UL-1, UL-3C). While cisplatin (15-75 μg/ml) induced apoptosis in monolayer and spheroid cultures, Taxol (100-800 nM) induced fragmentation in monolayers only. Cisplatin induced up to 5-fold DNA fragmentation in monolayers, while 3-fold (UL-3C, SKOV-3), and 1.5-fold (UL-1) in spheroids. Taxol treatment of monolayers resulted in the characteristic phosphorylation of Bcl-2, which was not demonstrated in spheroid cultures. Bax expression was reduced in spheroids following cisplatin or Taxol treatment, while p53 levels remained unchanged.

KW - Bcl-2

KW - Ovarian cancer

KW - Phosphorylation

KW - Spheroids

KW - Taxol

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Taxol-induced Bcl-2 phosphorylation in ovarian cancer cell monolayer and spheroids

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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