Taxol-induced Bcl-2 phosphorylation in ovarian cancer cell monolayer and spheroids

S. Makhija, D. D. Taylor, R. K. Gibb, Ç Gerçel-Taylor

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Expression of Bcl-2, Bax, p53 and induction of apoptosis were studied in cisplatin or Taxol treated monolayer and spheroid cultures of ovarian cancer cell lines (SKOV-3, UL-1, UL-3C). While cisplatin (15-75 μg/ml) induced apoptosis in monolayer and spheroid cultures, Taxol (100-800 nM) induced fragmentation in monolayers only. Cisplatin induced up to 5-fold DNA fragmentation in monolayers, while 3-fold (UL-3C, SKOV-3), and 1.5-fold (UL-1) in spheroids. Taxol treatment of monolayers resulted in the characteristic phosphorylation of Bcl-2, which was not demonstrated in spheroid cultures. Bax expression was reduced in spheroids following cisplatin or Taxol treatment, while p53 levels remained unchanged.

Original languageEnglish (US)
Pages (from-to)515-521
Number of pages7
JournalInternational journal of oncology
Volume14
Issue number3
StatePublished - Mar 1 1999
Externally publishedYes

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Keywords

  • Bcl-2
  • Ovarian cancer
  • Phosphorylation
  • Spheroids
  • Taxol

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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