Targeting of rough endoplasmic reticulum membrane proteins and ribosomes in invertebrate neurons

Melissa M. Rolls, David H. Hall, Martin Victor, Ernst H K Stelzer, Tom A. Rapoport

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The endoplasmic reticulum (ER) is divided into rough and smooth domains (RER and SER). The two domains share most proteins, but RER is enriched in some membrane proteins by an unknown mechanism. We studied RER protein targeting by expressing fluorescent protein fusions to ER membrane proteins in Caenorhabditis elegans. In several cell types RER and general ER proteins colocalized, but in neurons RER proteins were concentrated in the cell body, whereas general ER proteins were also found in neurites. Surprisingly RER membrane proteins diffused rapidly within the cell body, indicating they are not localized by immobilization. Ribosomes were also concentrated in the cell body, suggesting they may be in part responsible for targeting RER membrane proteins.

Original languageEnglish (US)
Pages (from-to)1778-1791
Number of pages14
JournalMolecular Biology of the Cell
Volume13
Issue number5
DOIs
StatePublished - 2002
Externally publishedYes

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Rough Endoplasmic Reticulum
Invertebrates
Ribosomes
Membrane Proteins
Endoplasmic Reticulum
Neurons
Proteins
Caenorhabditis elegans
Neurites
Protein Transport
Immobilization
Cell Body

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Targeting of rough endoplasmic reticulum membrane proteins and ribosomes in invertebrate neurons. / Rolls, Melissa M.; Hall, David H.; Victor, Martin; Stelzer, Ernst H K; Rapoport, Tom A.

In: Molecular Biology of the Cell, Vol. 13, No. 5, 2002, p. 1778-1791.

Research output: Contribution to journalArticle

Rolls, Melissa M. ; Hall, David H. ; Victor, Martin ; Stelzer, Ernst H K ; Rapoport, Tom A. / Targeting of rough endoplasmic reticulum membrane proteins and ribosomes in invertebrate neurons. In: Molecular Biology of the Cell. 2002 ; Vol. 13, No. 5. pp. 1778-1791.
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