Tandem transcription termination sites in the dnaN gene of Escherichia coli

M. E. Armengod, M. Garcia-Sogo, I. Perez-Roger, F. Macian, J. P. Navarro-Avino

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The dnaN gene of Escherichia coli encodes the β-subunit of DNA polymerase III and maps between the dnaA and recF genes. We demonstrated previously that dnaN and recF constitute a transcriptional unit under control of the dnaN promoters. However, the recF gene has its own promoter region located in the middle of the dnaN structural gene. In this report, we use S1 mapping of mRNAs, transcriptional and translational fusions to the galK and lacZ genes, and in vitro mutagenesis to identify and characterize three tandem transcription termination sites responsible for transcriptional polarity in the dnaN-recF operon. These sites are located in the dnaN gene, downstream from the recF promoter region. Cumulatively, they terminate about 80% of the untranslated transcripts started at the recF promoters. As expected, they do not reduce transcription coming from the dnaN promoters unless dnaN translation was prematurely disrupted by the presence of a nonsense codon. The particular arrangement of regulatory elements (promoters and terminators) in the dnaN-recF region provides an exceptional in vivo system to confirm the latent termination site model of transcriptional polarity. In addition, our results contribute to the understanding of the complex regulation of the dnaA, dnaN, and recF genes. We propose that these three genes constitute an operon and that the terminators described in this work could be used to reduce expression of the distal genes of the operon under circumstances in which the dnaN translation happens to be slowed down.

Original languageEnglish (US)
Pages (from-to)19725-19730
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number29
StatePublished - Jan 1 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Armengod, M. E., Garcia-Sogo, M., Perez-Roger, I., Macian, F., & Navarro-Avino, J. P. (1991). Tandem transcription termination sites in the dnaN gene of Escherichia coli. Journal of Biological Chemistry, 266(29), 19725-19730.