Tandem transcription termination sites in the dnaN gene of Escherichia coli

M. Eugenia Armengod, Maida García-Sogo, Ignacio Pérez-Roger, Fernando Macian-Juan, Juan P. Navarro-Aviñó

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The dnaN gene of Escherichia coli encodes the β-subunit of DNA polymerase III and maps between the dnaA and recF genes. We demonstrated previously that dnaN and recF constitute a transcriptional unit under control of the dnaN promoters. However, the recF gene has its own promoter region located in the middle of the dnaN structural gene. In this report, we use S1 mapping of mRNAs, transcriptional and translational fusions to the galK and lacZ genes, and in vitro mutagenesis to identify and characterize three tandem transcription termination sites responsible for transcriptional polarity in the dnaN-recF operon. These sites are located in the dnaN gene, downstream from the recF promoter region. Cumulatively, they terminate about 80% of the untranslated transcripts started at the recF promoters. As expected, they do not reduce transcription coming from the dnaN promoters unless dnaN translation was prematurely disrupted by the presence of a nonsense codon. The particular arrangement of regulatory elements (promoters and terminators) in the dnaN-recF region provides an exceptional in vivo system to confirm the latent termination site model of transcriptional polarity. In addition, our results contribute to the understanding of the complex regulation of the dnaA, dnaN, and recF genes. We propose that these three genes constitute an operon and that the terminators described in this work could be used to reduce expression of the distal genes of the operon under circumstances in which the dnaN translation happens to be slowed down.

Original languageEnglish (US)
Pages (from-to)19725-19730
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number29
StatePublished - Oct 15 1991
Externally publishedYes

Fingerprint

Transcription
Escherichia coli
Genes
Operon
Genetic Promoter Regions
DNA Polymerase III
Lac Operon
Nonsense Codon
Mutagenesis
Gene Expression
Messenger RNA
Fusion reactions

ASJC Scopus subject areas

  • Biochemistry

Cite this

Armengod, M. E., García-Sogo, M., Pérez-Roger, I., Macian-Juan, F., & Navarro-Aviñó, J. P. (1991). Tandem transcription termination sites in the dnaN gene of Escherichia coli. Journal of Biological Chemistry, 266(29), 19725-19730.

Tandem transcription termination sites in the dnaN gene of Escherichia coli. / Armengod, M. Eugenia; García-Sogo, Maida; Pérez-Roger, Ignacio; Macian-Juan, Fernando; Navarro-Aviñó, Juan P.

In: Journal of Biological Chemistry, Vol. 266, No. 29, 15.10.1991, p. 19725-19730.

Research output: Contribution to journalArticle

Armengod, ME, García-Sogo, M, Pérez-Roger, I, Macian-Juan, F & Navarro-Aviñó, JP 1991, 'Tandem transcription termination sites in the dnaN gene of Escherichia coli', Journal of Biological Chemistry, vol. 266, no. 29, pp. 19725-19730.
Armengod ME, García-Sogo M, Pérez-Roger I, Macian-Juan F, Navarro-Aviñó JP. Tandem transcription termination sites in the dnaN gene of Escherichia coli. Journal of Biological Chemistry. 1991 Oct 15;266(29):19725-19730.
Armengod, M. Eugenia ; García-Sogo, Maida ; Pérez-Roger, Ignacio ; Macian-Juan, Fernando ; Navarro-Aviñó, Juan P. / Tandem transcription termination sites in the dnaN gene of Escherichia coli. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 29. pp. 19725-19730.
@article{1277877755664cd18244be18fd1e97cb,
title = "Tandem transcription termination sites in the dnaN gene of Escherichia coli",
abstract = "The dnaN gene of Escherichia coli encodes the β-subunit of DNA polymerase III and maps between the dnaA and recF genes. We demonstrated previously that dnaN and recF constitute a transcriptional unit under control of the dnaN promoters. However, the recF gene has its own promoter region located in the middle of the dnaN structural gene. In this report, we use S1 mapping of mRNAs, transcriptional and translational fusions to the galK and lacZ genes, and in vitro mutagenesis to identify and characterize three tandem transcription termination sites responsible for transcriptional polarity in the dnaN-recF operon. These sites are located in the dnaN gene, downstream from the recF promoter region. Cumulatively, they terminate about 80{\%} of the untranslated transcripts started at the recF promoters. As expected, they do not reduce transcription coming from the dnaN promoters unless dnaN translation was prematurely disrupted by the presence of a nonsense codon. The particular arrangement of regulatory elements (promoters and terminators) in the dnaN-recF region provides an exceptional in vivo system to confirm the latent termination site model of transcriptional polarity. In addition, our results contribute to the understanding of the complex regulation of the dnaA, dnaN, and recF genes. We propose that these three genes constitute an operon and that the terminators described in this work could be used to reduce expression of the distal genes of the operon under circumstances in which the dnaN translation happens to be slowed down.",
author = "Armengod, {M. Eugenia} and Maida Garc{\'i}a-Sogo and Ignacio P{\'e}rez-Roger and Fernando Macian-Juan and Navarro-Avi{\~n}{\'o}, {Juan P.}",
year = "1991",
month = "10",
day = "15",
language = "English (US)",
volume = "266",
pages = "19725--19730",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "29",

}

TY - JOUR

T1 - Tandem transcription termination sites in the dnaN gene of Escherichia coli

AU - Armengod, M. Eugenia

AU - García-Sogo, Maida

AU - Pérez-Roger, Ignacio

AU - Macian-Juan, Fernando

AU - Navarro-Aviñó, Juan P.

PY - 1991/10/15

Y1 - 1991/10/15

N2 - The dnaN gene of Escherichia coli encodes the β-subunit of DNA polymerase III and maps between the dnaA and recF genes. We demonstrated previously that dnaN and recF constitute a transcriptional unit under control of the dnaN promoters. However, the recF gene has its own promoter region located in the middle of the dnaN structural gene. In this report, we use S1 mapping of mRNAs, transcriptional and translational fusions to the galK and lacZ genes, and in vitro mutagenesis to identify and characterize three tandem transcription termination sites responsible for transcriptional polarity in the dnaN-recF operon. These sites are located in the dnaN gene, downstream from the recF promoter region. Cumulatively, they terminate about 80% of the untranslated transcripts started at the recF promoters. As expected, they do not reduce transcription coming from the dnaN promoters unless dnaN translation was prematurely disrupted by the presence of a nonsense codon. The particular arrangement of regulatory elements (promoters and terminators) in the dnaN-recF region provides an exceptional in vivo system to confirm the latent termination site model of transcriptional polarity. In addition, our results contribute to the understanding of the complex regulation of the dnaA, dnaN, and recF genes. We propose that these three genes constitute an operon and that the terminators described in this work could be used to reduce expression of the distal genes of the operon under circumstances in which the dnaN translation happens to be slowed down.

AB - The dnaN gene of Escherichia coli encodes the β-subunit of DNA polymerase III and maps between the dnaA and recF genes. We demonstrated previously that dnaN and recF constitute a transcriptional unit under control of the dnaN promoters. However, the recF gene has its own promoter region located in the middle of the dnaN structural gene. In this report, we use S1 mapping of mRNAs, transcriptional and translational fusions to the galK and lacZ genes, and in vitro mutagenesis to identify and characterize three tandem transcription termination sites responsible for transcriptional polarity in the dnaN-recF operon. These sites are located in the dnaN gene, downstream from the recF promoter region. Cumulatively, they terminate about 80% of the untranslated transcripts started at the recF promoters. As expected, they do not reduce transcription coming from the dnaN promoters unless dnaN translation was prematurely disrupted by the presence of a nonsense codon. The particular arrangement of regulatory elements (promoters and terminators) in the dnaN-recF region provides an exceptional in vivo system to confirm the latent termination site model of transcriptional polarity. In addition, our results contribute to the understanding of the complex regulation of the dnaA, dnaN, and recF genes. We propose that these three genes constitute an operon and that the terminators described in this work could be used to reduce expression of the distal genes of the operon under circumstances in which the dnaN translation happens to be slowed down.

UR - http://www.scopus.com/inward/record.url?scp=0025985503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025985503&partnerID=8YFLogxK

M3 - Article

C2 - 1918078

AN - SCOPUS:0025985503

VL - 266

SP - 19725

EP - 19730

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 29

ER -