Syntabulin, a motor protein linker, controls dorsal determination

Hideaki Nojima, Sophie Rothhämel, Takashi Shimizu, Cheol Hee Kim, Shigenobu Yonemura, Florence L. Marlow, Masahiko Hibi

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

In amphibian and teleost embryos, the dorsal determinants (DDs) are believed to be initially localized to the vegetal pole and then transported to the prospective dorsal side of the embryo along a microtubule array. The DDs are known to activate the canonical Wnt pathway and thereby promote the expression of genes that induce the dorsal organizer. Here, by identifying the locus of the maternal-effect ventralized mutant tokkaebi, we show that Syntabulin, a linker of the kinesin I motor protein, is essential for dorsal determination in zebrafish. We found that syntabulin mRNA is transported to the vegetal pole during oogenesis through the Bucky ball (Buc)-mediated Balbiani body-dependent pathway, which is necessary for establishment of animal-vegetal (AV) oocyte polarity. We demonstrate that Syntabulin is translocated from the vegetal pole in a microtubule-dependent manner. Our findings suggest that Syntabulin regulates the microtubule-dependent transport of the DDs, and provide evidence for the link between AV and dorsoventral axis formation.

Original languageEnglish (US)
Pages (from-to)923-933
Number of pages11
JournalDevelopment
Volume137
Issue number6
DOIs
StatePublished - Mar 15 2010

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Microtubules
Embryonic Structures
Kinesin
Oogenesis
Wnt Signaling Pathway
Proteins
Amphibians
Zebrafish
Oocytes
Gene Expression
Messenger RNA
Maternal Inheritance

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Nojima, H., Rothhämel, S., Shimizu, T., Kim, C. H., Yonemura, S., Marlow, F. L., & Hibi, M. (2010). Syntabulin, a motor protein linker, controls dorsal determination. Development, 137(6), 923-933. https://doi.org/10.1242/dev.046425

Syntabulin, a motor protein linker, controls dorsal determination. / Nojima, Hideaki; Rothhämel, Sophie; Shimizu, Takashi; Kim, Cheol Hee; Yonemura, Shigenobu; Marlow, Florence L.; Hibi, Masahiko.

In: Development, Vol. 137, No. 6, 15.03.2010, p. 923-933.

Research output: Contribution to journalArticle

Nojima, H, Rothhämel, S, Shimizu, T, Kim, CH, Yonemura, S, Marlow, FL & Hibi, M 2010, 'Syntabulin, a motor protein linker, controls dorsal determination', Development, vol. 137, no. 6, pp. 923-933. https://doi.org/10.1242/dev.046425
Nojima H, Rothhämel S, Shimizu T, Kim CH, Yonemura S, Marlow FL et al. Syntabulin, a motor protein linker, controls dorsal determination. Development. 2010 Mar 15;137(6):923-933. https://doi.org/10.1242/dev.046425
Nojima, Hideaki ; Rothhämel, Sophie ; Shimizu, Takashi ; Kim, Cheol Hee ; Yonemura, Shigenobu ; Marlow, Florence L. ; Hibi, Masahiko. / Syntabulin, a motor protein linker, controls dorsal determination. In: Development. 2010 ; Vol. 137, No. 6. pp. 923-933.
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