Synergism between hemopoietic growth factors (HGFs) detected by their effects on cells bearing receptors for a lineage specific HGF

Assay of hemopoietin-1

S. H. Bartelmez, E. R. Stanley

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The preceding paper describes a new approach to the detection and assay of growth factors for developmentally early multipotent hemopoietic cells. This approach, involving measurement of the increase in the number of receptors for the mononuclear phagocyte specific hemopoietic growth factor (HGF), colony stimulating factor-1 (CSF-1), in cultures of developmentally early murine cells incubated with putative HGFs, has been used to define and assay hemopoietin-1. Hemopoietin-1 (M(r) ~ 20,000) is found in the medium derived from serum-free cultures of cells of the human urinary bladder carcinoma line 5637. In contrast to both hemopoietin-2 and CSF-1, which also stimulate an increase in CSF-1 receptor numbers in cultures of developmentally early hemopoietic cells, hemopoietin-1 alone has no detectable effect. However, hemopoietin-1 exhibits dramatic synergism with CSF-1. In the presence of CSF-1, hemopoietin-1 stimulates the proliferation of developmentally earlier cells than those that respond to either CSF-1 alone or hemopoietin-2 alone or their combination. These cells proliferate for at least 3 days with no alteration of the average CSF-1 receptor density. However, by 5 days of incubation, the progeny of developmentally early hemopoietic cells that have proliferated in response to hemopoietin-1 + CSF-1 exhibit an approximately tenfold increase in the average CSF-1 receptor density per cell, which immediately precedes their differentiation to adherent mononuclear phagocytes. As hemopoietin-1 does not possess colony stimulating or burst promoting activities for murine bone marrow cells, but acts on multipotent hemopoietin cells, the analysis of the mechanism of its synergistic effects with HGFs such as CSF-1 are of special relevance to the regulation of early events in hemopoiesis.

Original languageEnglish (US)
Pages (from-to)370-378
Number of pages9
JournalJournal of Cellular Physiology
Volume122
Issue number3
StatePublished - 1985

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Bearings (structural)
Macrophage Colony-Stimulating Factor
Assays
Intercellular Signaling Peptides and Proteins
Colony-Stimulating Factor Receptors
Cells
Phagocytes
Serum-Free Culture Media
Cell culture
Bone Marrow Cells
Bone
Urinary Bladder

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Synergism between hemopoietic growth factors (HGFs) detected by their effects on cells bearing receptors for a lineage specific HGF : Assay of hemopoietin-1. / Bartelmez, S. H.; Stanley, E. R.

In: Journal of Cellular Physiology, Vol. 122, No. 3, 1985, p. 370-378.

Research output: Contribution to journalArticle

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