Syndapin/SDPN-1 is required for endocytic recycling and endosomal actin association in the Caenorhabditis elegans intestine

Adenrele M. Gleason, Ken C.Q. Nguyen, David H. Hall, Barth D. Grant

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Syndapin/pascin-family F-BAR domain proteins bind directly to membrane lipids and are associated with actin dynamics at the plasma membrane. Previous reports also implicated mammalian syndapin 2 in endosome function during receptor recycling, but precise analysis of a putative recycling function for syndapin in mammalian systems is difficult because of its effects on the earlier step of endocytic uptake and potential redundancy among the three separate genes that encode mammalian syndapin isoforms. Here we analyze the endocytic transport function of the only Caenorhabditis elegans syndapin, SDPN-1. We find that SDPN-1 is a resident protein of the early and basolateral recycling endosomes in the C. elegans intestinal epithelium, and sdpn-1 deletion mutants display phenotypes indicating a block in basolateral recycling transport. sdpn-1 mutants accumulate abnormal endosomes positive for early endosome and recycling endosome markers that are normally separate, and such endosomes accumulate high levels of basolateral recycling cargo. Furthermore, we observed strong colocalization of endosomal SDPN-1 with the F-Actin biosensor Lifeact and found that loss of SDPN-1 greatly reduced Lifeact accumulation on early endosomes. Taken together, our results provide strong evidence for an in vivo function of syndapin in endocytic recycling and suggest that syndapin promotes transport via endosomal fission.

Original languageEnglish (US)
Pages (from-to)3746-3756
Number of pages11
JournalMolecular Biology of the Cell
Volume27
Issue number23
DOIs
StatePublished - Nov 15 2016

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Endosomes
Caenorhabditis elegans
Intestines
Actins
Recycling
Biosensing Techniques
Intestinal Mucosa
Membrane Lipids
Protein Isoforms
Cell Membrane
Phenotype
Genes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Syndapin/SDPN-1 is required for endocytic recycling and endosomal actin association in the Caenorhabditis elegans intestine. / Gleason, Adenrele M.; Nguyen, Ken C.Q.; Hall, David H.; Grant, Barth D.

In: Molecular Biology of the Cell, Vol. 27, No. 23, 15.11.2016, p. 3746-3756.

Research output: Contribution to journalArticle

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