TY - JOUR
T1 - Studying protein dynamics in living cells
AU - Lippincott-Schwartz, Jennifer
AU - Snapp, Erik
AU - Kemvorthy, Anne
PY - 2001/6
Y1 - 2001/6
N2 - Since the advent of the green fluorescent protein, the subcellular localization, mobility, transport routes and binding interactions of proteins can be studied in living cells. Live cell imaging, in combination with photobleaching, energy transfer or fluorescence correlation spectroscopy are providing unprecedented insights into the movement of proteins and their interactions with cellular components. Remarkably, these powerful techniques are accessible to non-specialists using commercially available microscope systems.
AB - Since the advent of the green fluorescent protein, the subcellular localization, mobility, transport routes and binding interactions of proteins can be studied in living cells. Live cell imaging, in combination with photobleaching, energy transfer or fluorescence correlation spectroscopy are providing unprecedented insights into the movement of proteins and their interactions with cellular components. Remarkably, these powerful techniques are accessible to non-specialists using commercially available microscope systems.
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U2 - 10.1038/35073068
DO - 10.1038/35073068
M3 - Review article
C2 - 11389468
AN - SCOPUS:0035374872
SN - 1471-0072
VL - 2
SP - 444
EP - 456
JO - Nature Reviews Molecular Cell Biology
JF - Nature Reviews Molecular Cell Biology
IS - 6
ER -