Studying protein dynamics in living cells

Jennifer Lippincott-Schwartz, Erik Snapp, Anne Kemvorthy

Research output: Contribution to journalReview article

900 Scopus citations

Abstract

Since the advent of the green fluorescent protein, the subcellular localization, mobility, transport routes and binding interactions of proteins can be studied in living cells. Live cell imaging, in combination with photobleaching, energy transfer or fluorescence correlation spectroscopy are providing unprecedented insights into the movement of proteins and their interactions with cellular components. Remarkably, these powerful techniques are accessible to non-specialists using commercially available microscope systems.

Original languageEnglish (US)
Pages (from-to)444-456
Number of pages13
JournalNature Reviews Molecular Cell Biology
Volume2
Issue number6
DOIs
StatePublished - Jun 1 2001

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Lippincott-Schwartz, J., Snapp, E., & Kemvorthy, A. (2001). Studying protein dynamics in living cells. Nature Reviews Molecular Cell Biology, 2(6), 444-456. https://doi.org/10.1038/35073068