TY - JOUR
T1 - Studies on the biological characterization and mitogenic interactions between hepatic stimulator substance and acidic fibroblast growth factor
AU - Gupta, Sanjeev
AU - Kan, Mikio
AU - Vemuru, Ravikumar P.
AU - Labrecque, Douglas R.
AU - McKeehan, Wallace L.
PY - 1994/4/1
Y1 - 1994/4/1
N2 - During liver regeneration, hepatic stimulator substance (HSS) and acidic fibroblast growth factor (FGF-1) are produced in the liver. These growth factors may be involved in liver growth control but an understanding of their regulatory interactions is limited. To further characterize the mitogenic activity of HSS, we compared its effects with FGF-1 in cells of hepatocyte, non-parenchymal liver epithelial and non-hepatic lineages. Our studies with these cell types demonstrated differences in the mitogenic specificities of HSS and FGF-1. Whereas exposure of primary hepatocytes to epidermal growth factor and HSS synergistically increased DNA synthesis, simultaneous exposure to HSS and FGF-1 resulted in no such effect. Receptor-binding assays showed that HSS did not compete with FGF-1 in binding to FGF-1 receptors on rat primary hepatocytes. Additional immunoblot analysis demonstrated no cross-reactivity between FGF-1 antibodies and HSS. Distinct mitogenic and immunologic properties of HSS and FGF-1 should facilitate further analysis of liver regeneration and hepatic oncogenesis.
AB - During liver regeneration, hepatic stimulator substance (HSS) and acidic fibroblast growth factor (FGF-1) are produced in the liver. These growth factors may be involved in liver growth control but an understanding of their regulatory interactions is limited. To further characterize the mitogenic activity of HSS, we compared its effects with FGF-1 in cells of hepatocyte, non-parenchymal liver epithelial and non-hepatic lineages. Our studies with these cell types demonstrated differences in the mitogenic specificities of HSS and FGF-1. Whereas exposure of primary hepatocytes to epidermal growth factor and HSS synergistically increased DNA synthesis, simultaneous exposure to HSS and FGF-1 resulted in no such effect. Receptor-binding assays showed that HSS did not compete with FGF-1 in binding to FGF-1 receptors on rat primary hepatocytes. Additional immunoblot analysis demonstrated no cross-reactivity between FGF-1 antibodies and HSS. Distinct mitogenic and immunologic properties of HSS and FGF-1 should facilitate further analysis of liver regeneration and hepatic oncogenesis.
KW - Cell proliferation
KW - Fibroblast growth factors
KW - Heparin-binding growth factors
KW - Hepatic growth factors
KW - Liver
KW - Liver regeneration
UR - http://www.scopus.com/inward/record.url?scp=0028260123&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028260123&partnerID=8YFLogxK
U2 - 10.1016/0304-3835(94)90035-3
DO - 10.1016/0304-3835(94)90035-3
M3 - Article
C2 - 7514091
AN - SCOPUS:0028260123
SN - 0304-3835
VL - 78
SP - 85
EP - 92
JO - Cancer Letters
JF - Cancer Letters
IS - 1-3
ER -