Studies of a ring-cleaving dioxygenase illuminate the role of cholesterol metabolism in the pathogenesis of Mycobacterium tuberculosis

Katherine C. Yam, Igor D'Angelo, Rainer Kalscheuer, Haizhong Zhu, Jian Xin Wang, Victor Snieckus, Lan H. Ly, Paul J. Converse, William R. Jacobs, Natalie Strynadka, Lindsay D. Eltis

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Mycobacterium tuberculosis, the etiological agent of TB, possesses a cholesterol catabolic pathway implicated in pathogenesis. This pathway includes an iron-dependent extradiol dioxygenase, HsaC, that cleaves catechols. Immunocompromised mice infected with a ΔhsaC mutant of M. tuberculosis H37Rv survived 50% longer than mice infected with the wild-type strain. In guinea pigs, the mutant disseminated more slowly to the spleen, persisted less successfully in the lung, and caused little pathology. These data establish that, while cholesterol metabolism by M. tuberculosis appears to be most important during the chronic stage of infection, it begins much earlier and may contribute to the pathogen's dissemination within the host. Purified HsaC efficiently cleaved the catecholic cholesterol metabolite, DHSA (3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione;k cat/Km = 14.4±0.5 μM-1 s -1), and was inactivated by a halogenated substrate analogue (partition coefficient<50). Remarkably, cholesterol caused loss of viability in the ΔhsaC mutant, consistent with catechol toxicity. Structures of HsaC:DHSA binary complexes at 2.1 Å revealed two catechol-binding modes: bidentate binding to the active site iron, as has been reported in similar enzymes, and, unexpectedly, monodentate binding. The position of the bicyclo-alkanone moiety of DHSA was very similar in the two binding modes, suggesting that this interaction is a determinant in the initial substrate-binding event. These data provide insights into the binding of catechols by extradiol dioxygenases and facilitate inhibitor design.

Original languageEnglish (US)
JournalPLoS pathogens
Volume5
Issue number3
DOIs
StatePublished - Mar 1 2009

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Dioxygenases
Mycobacterium tuberculosis
Cholesterol
Catechols
Iron
Catalytic Domain
Guinea Pigs
Cats
Spleen
Pathology
Lung
Enzymes
Infection
extradiol dioxygenase
catechol

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Studies of a ring-cleaving dioxygenase illuminate the role of cholesterol metabolism in the pathogenesis of Mycobacterium tuberculosis. / Yam, Katherine C.; D'Angelo, Igor; Kalscheuer, Rainer; Zhu, Haizhong; Wang, Jian Xin; Snieckus, Victor; Ly, Lan H.; Converse, Paul J.; Jacobs, William R.; Strynadka, Natalie; Eltis, Lindsay D.

In: PLoS pathogens, Vol. 5, No. 3, 01.03.2009.

Research output: Contribution to journalArticle

Yam, KC, D'Angelo, I, Kalscheuer, R, Zhu, H, Wang, JX, Snieckus, V, Ly, LH, Converse, PJ, Jacobs, WR, Strynadka, N & Eltis, LD 2009, 'Studies of a ring-cleaving dioxygenase illuminate the role of cholesterol metabolism in the pathogenesis of Mycobacterium tuberculosis', PLoS pathogens, vol. 5, no. 3. https://doi.org/10.1371/journal.ppat.1000344
Yam, Katherine C. ; D'Angelo, Igor ; Kalscheuer, Rainer ; Zhu, Haizhong ; Wang, Jian Xin ; Snieckus, Victor ; Ly, Lan H. ; Converse, Paul J. ; Jacobs, William R. ; Strynadka, Natalie ; Eltis, Lindsay D. / Studies of a ring-cleaving dioxygenase illuminate the role of cholesterol metabolism in the pathogenesis of Mycobacterium tuberculosis. In: PLoS pathogens. 2009 ; Vol. 5, No. 3.
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AU - Wang, Jian Xin

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AU - Ly, Lan H.

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