Structures of three distinct activator-TFIID complexes

Wei Li Liu, Robert A. Coleman, Elizabeth Ma, Patricia Grob, Joyce L. Yang, Yixi Zhang, Gina Dailey, Eva Nogales, Robert Tjian

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Sequence-specific DNA-binding activators, key regulators of gene expression, stimulate transcription in part by targeting the core promoter recognition TFIID complex and aiding in its recruitment to promoter DNA. Although it has been established that activators can interact with multiple components of TFIID, it is unknown whether common or distinct surfaces within TFIID are targeted by activators and what changes if any in the structure of TFIID may occur upon binding activators. As a first step toward structurally dissecting activator/TFIID interactions, we determined the three-dimensional structures of TFIID bound to three distinct activators (i.e., the tumor suppressor p53 protein, glutamine-rich Sp1 and the oncoprotein c-Jun) and compared their structures as determined by electron microscopy and single-particle reconstruction. By a combination of EM and biochemical mapping analysis, our results uncover distinct contact regions within TFIID bound by each activator. Unlike the coactivator CRSP/Mediator complex that undergoes drastic and global structural changes upon activator binding, instead, a rather confined set of local conserved structural changes were observed when each activator binds holo-TFIID. These results suggest that activator contact may induce unique structural features of TFIID, thus providing nanoscale information on activator-dependent TFIID assembly and transcription initiation.

Original languageEnglish (US)
Pages (from-to)1510-1521
Number of pages12
JournalGenes and Development
Volume23
Issue number13
DOIs
StatePublished - Jul 1 2009

Keywords

  • Activator
  • Structure
  • TAF
  • TFIID
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Fingerprint Dive into the research topics of 'Structures of three distinct activator-TFIID complexes'. Together they form a unique fingerprint.

  • Cite this

    Liu, W. L., Coleman, R. A., Ma, E., Grob, P., Yang, J. L., Zhang, Y., Dailey, G., Nogales, E., & Tjian, R. (2009). Structures of three distinct activator-TFIID complexes. Genes and Development, 23(13), 1510-1521. https://doi.org/10.1101/gad.1790709