Structure-function studies of a colony stimulating factor (CSF-1)

S. K. Das, E. R. Stanley

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

CSF-1 is a glycoprotein growth factor which specifically stimulates the survival, proliferation, and differentiation of cells of the mononuclear phagocytic lineage. In this study, microgram amounts of radiolabeled murine L-cell and human urinary CSF-1 were isolated in pure form and used to investigate the nature and extent of CSF-1 glycosylation and the requirment of the carbohydrate moiety for its biological and antibody-binding activities. The molecular weight of the preparations examined varied betwen ~ 47,000 and ~ 76,000. Reduction and alkylation halved the molecular weight of all preparations, reflecting within each the existence of two similar subunits. Exhaustive treatment of reduced and alkylated CSF-1 with endo-β-N-acetylglucosaminidase D, but not endo-β-N-acetylglucosamindase H, gave rise to a molecular of M(r) ~ 16,500 of which the polypeptide portion accounted for ~ 14,500. Whereas the parent molecule bound concanavalin A, this product did not, indicating that heterogeneity in the saccharide component could explain the observed variation in the molecular weight of CSF-1. These results also suggest that the two polypeptide chains in the dimeric CSF-1 molecule are very similar and posibly even identical. Because of the specificity of endo-β-N-acetylglucosaminidase D, it is concluded that the carbohydrate moieties are Asn-lined 'complex-type' units. Examination of the effects of endo-α-N-acetyl-D-galactosaminidase treatment on the molecular weight of reduced and alkylated CSF-2 failed to provide evidence for the existence of O-glycosidically linked oligosaccharides. Treatment of native CSF-1 with endo-β-N-acetylglucosaminidase D was almost as efficient in removing carbohydrate as in the case of the reduced and alkylated subunits. Removal did not cause loss of antibody binding, receptor binding, or biological activity.

Original languageEnglish (US)
Pages (from-to)13679-13684
Number of pages6
JournalJournal of Biological Chemistry
Volume257
Issue number22
StatePublished - 1982

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Colony-Stimulating Factors
Macrophage Colony-Stimulating Factor
Acetylglucosaminidase
Molecular Weight
Molecular weight
Carbohydrates
Glycosylation
Hexosaminidases
Peptides
Molecules
Antibodies
Alkylation
Granulocyte-Macrophage Colony-Stimulating Factor
Phagocytes
Concanavalin A
Bioactivity
Oligosaccharides
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Glycoproteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure-function studies of a colony stimulating factor (CSF-1). / Das, S. K.; Stanley, E. R.

In: Journal of Biological Chemistry, Vol. 257, No. 22, 1982, p. 13679-13684.

Research output: Contribution to journalArticle

Das, SK & Stanley, ER 1982, 'Structure-function studies of a colony stimulating factor (CSF-1)', Journal of Biological Chemistry, vol. 257, no. 22, pp. 13679-13684.
Das, S. K. ; Stanley, E. R. / Structure-function studies of a colony stimulating factor (CSF-1). In: Journal of Biological Chemistry. 1982 ; Vol. 257, No. 22. pp. 13679-13684.
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