Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes

Charalampos G. Kalodimos, Nikolaos Biris, Alexandre M J J Bonvin, Marc M. Levandoski, Marc Guennuegues, Rolf Boelens, Robert Kaptein

Research output: Contribution to journalArticle

377 Citations (Scopus)

Abstract

Interaction of regulatory DNA binding proteins with their target sites is usually preceded by binding to nonspecific DNA. This speeds up the search for the target site by several orders of magnitude. We report the solution structure and dynamics of the complex of a dimeric lac repressor DNA binding domain with nonspecific DNA. The same set of residues can switch roles from a purely electrostatic interaction with the DNA backbone in the nonspecific complex to a highly specific binding mode with the base pairs of the cognate operator sequence. The protein-DNA interface of the nonspecific complex is flexible on biologically relevant time scales that may assist in the rapid and efficient finding of the target site.

Original languageEnglish (US)
Pages (from-to)386-389
Number of pages4
JournalScience
Volume305
Issue number5682
DOIs
StatePublished - Jul 16 2004
Externally publishedYes

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DNA
Proteins
Lac Repressors
DNA-Binding Proteins
Static Electricity
Base Pairing

ASJC Scopus subject areas

  • General

Cite this

Kalodimos, C. G., Biris, N., Bonvin, A. M. J. J., Levandoski, M. M., Guennuegues, M., Boelens, R., & Kaptein, R. (2004). Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes. Science, 305(5682), 386-389. https://doi.org/10.1126/science.1097064

Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes. / Kalodimos, Charalampos G.; Biris, Nikolaos; Bonvin, Alexandre M J J; Levandoski, Marc M.; Guennuegues, Marc; Boelens, Rolf; Kaptein, Robert.

In: Science, Vol. 305, No. 5682, 16.07.2004, p. 386-389.

Research output: Contribution to journalArticle

Kalodimos, CG, Biris, N, Bonvin, AMJJ, Levandoski, MM, Guennuegues, M, Boelens, R & Kaptein, R 2004, 'Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes', Science, vol. 305, no. 5682, pp. 386-389. https://doi.org/10.1126/science.1097064
Kalodimos CG, Biris N, Bonvin AMJJ, Levandoski MM, Guennuegues M, Boelens R et al. Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes. Science. 2004 Jul 16;305(5682):386-389. https://doi.org/10.1126/science.1097064
Kalodimos, Charalampos G. ; Biris, Nikolaos ; Bonvin, Alexandre M J J ; Levandoski, Marc M. ; Guennuegues, Marc ; Boelens, Rolf ; Kaptein, Robert. / Structure and flexibility adaptation in nonspecific and specific protein-DNA complexes. In: Science. 2004 ; Vol. 305, No. 5682. pp. 386-389.
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