Structure and expression of the Caenorhabditis elegans protein kinase C2 gene. Origins and regulated expression of a family of Ca2+ -activated protein kinase C isoforms

Alma Islas-Trejo, Marianne Land, Irina Tcherepanova, Jonathan H. Freedman, Charles S. Rubin

Research output: Contribution to journalArticle

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Abstract

The molecular and cellular basis for concerted Ca2+/lipid signaling in Caenorhabditis elegans was investigated. A unique gene (pkc-2) and cognate cDNAs that encode six Ca2+/diacylglycerol-stimulated PKC2 isoenzymes were characterized. PKC2 polypeptides (680-717 amino acid residues) share identical catalytic, Ca2+ binding, diacylglycerol-activation and pseudosubstrate domains. However, sequences of the N- and C-terminal regions of the kinases diverge. PKC2 diversity is partly due to differential activation of transcription by distinct promoters. Each promoter precedes an adjacent exon that encodes 5'-untranslated RNA, an initiator AUG codon and a unique open reading frame. PKC2 mRNAs also incorporate one of two 3'- terminal exons via alternative splicing. Cells that are capable of receiving and propagating signals carried by Ca2+/diacylglyceroI were identified by assessing activities of pkc-2 gene promoters in transgenie C. elegans and visualizing the distribution of PKC2 polypeptides via immunofluorescence. Highly-selective expression of certain PKC2 isoforms was observed in distinct subsets of neurons, intestinal and muscle cells. A low level of PKC2 isoforms is observed in embryos. When L1 larvae hatch and interact with the external environment PKC2 content increases 10-fold. Although 77- and 78-kDa PKC2 isoforms are evident throughout post-embryonic development, an 81-kDa isoform appears to be adapted for function in L1 and L2 larvae.

Original languageEnglish (US)
Pages (from-to)6629-6640
Number of pages12
JournalJournal of Biological Chemistry
Volume272
Issue number10
DOIs
StatePublished - Mar 7 1997

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Caenorhabditis elegans Proteins
Protein Kinases
Protein Kinase C
Protein Isoforms
Genes
Diglycerides
Caenorhabditis elegans
Larva
Exons
Chemical activation
Hatches
Untranslated RNA
Peptides
Initiator Codon
Alternative Splicing
Transcription
Muscle Cells
Transcriptional Activation
Open Reading Frames
Isoenzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure and expression of the Caenorhabditis elegans protein kinase C2 gene. Origins and regulated expression of a family of Ca2+ -activated protein kinase C isoforms. / Islas-Trejo, Alma; Land, Marianne; Tcherepanova, Irina; Freedman, Jonathan H.; Rubin, Charles S.

In: Journal of Biological Chemistry, Vol. 272, No. 10, 07.03.1997, p. 6629-6640.

Research output: Contribution to journalArticle

Islas-Trejo, Alma ; Land, Marianne ; Tcherepanova, Irina ; Freedman, Jonathan H. ; Rubin, Charles S. / Structure and expression of the Caenorhabditis elegans protein kinase C2 gene. Origins and regulated expression of a family of Ca2+ -activated protein kinase C isoforms. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 10. pp. 6629-6640.
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