Structure and expression of a novel, neuronal protein kinase C (PKC1B) from Caenorhabditis elegans: PKC1B is expressed selectively in neurons that receive, transmit, and process environmental signals

Marianne Land, Alma Islas-Trejo, Jonathan H. Freedman, Charles S. Rubin

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The nematode Caenorhabditis elegans provides an advantageous system for investigating the regulation, expression, and functions of protein kinase C (PKC) isoforms. We cloned and characterized cDNAs encoding a novel C. elegans PKC designated PKC1B. The predicted PKC1B polypeptide contains features characteristic of the nPKC subfamily of PKC isoforms. The levels of PKC1B and its cognate mRNA vary over a 7-fold range during C. elegans postembryonic development. PKC1B protein and mRNA are abundant at the earliest larval stage, but their relative concentrations decrease coordinately in late larvae. Embryos, which are enriched in PKC1B mRNA, contain little PKC1B protein. Thus, PKC1B expression is regulated at a translational or post-translational level during early development. Cells engaged in PKC1B gene transcription were identified in transgenic C. elegans that carry the lacZ gene under the regulation of the PKC1B promoter. Staining for β-galactosidase revealed PKC1B promoter activity exclusively in sensory neurons and interneurons. Immunofluorescence microscopy disclosed that the PKC1B polypeptide is located in the processes (axons and dendrites) and perinuclear regions of ∼ 75 neurons that constitute the sensory circuitry of the nematode. The intracellular localization of PKC1B and the enzyme's differential solubility in ionic and nonionic detergents suggest that the kinase is associated with membranes and the cytoskeleton.

Original languageEnglish (US)
Pages (from-to)9234-9244
Number of pages11
JournalJournal of Biological Chemistry
Volume269
Issue number12
StatePublished - Mar 25 1994

Fingerprint

Caenorhabditis elegans
Protein Kinase C
Neurons
Sensory Receptor Cells
Messenger RNA
Protein Isoforms
Genes
Caenorhabditis elegans Proteins
Galactosidases
Peptides
Lac Operon
Interneurons
Transcription
Dendrites
Cytoskeleton
Fluorescence Microscopy
Detergents
Solubility
Larva
Axons

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure and expression of a novel, neuronal protein kinase C (PKC1B) from Caenorhabditis elegans : PKC1B is expressed selectively in neurons that receive, transmit, and process environmental signals. / Land, Marianne; Islas-Trejo, Alma; Freedman, Jonathan H.; Rubin, Charles S.

In: Journal of Biological Chemistry, Vol. 269, No. 12, 25.03.1994, p. 9234-9244.

Research output: Contribution to journalArticle

@article{880a8d925f864477b02e49a565d62dea,
title = "Structure and expression of a novel, neuronal protein kinase C (PKC1B) from Caenorhabditis elegans: PKC1B is expressed selectively in neurons that receive, transmit, and process environmental signals",
abstract = "The nematode Caenorhabditis elegans provides an advantageous system for investigating the regulation, expression, and functions of protein kinase C (PKC) isoforms. We cloned and characterized cDNAs encoding a novel C. elegans PKC designated PKC1B. The predicted PKC1B polypeptide contains features characteristic of the nPKC subfamily of PKC isoforms. The levels of PKC1B and its cognate mRNA vary over a 7-fold range during C. elegans postembryonic development. PKC1B protein and mRNA are abundant at the earliest larval stage, but their relative concentrations decrease coordinately in late larvae. Embryos, which are enriched in PKC1B mRNA, contain little PKC1B protein. Thus, PKC1B expression is regulated at a translational or post-translational level during early development. Cells engaged in PKC1B gene transcription were identified in transgenic C. elegans that carry the lacZ gene under the regulation of the PKC1B promoter. Staining for β-galactosidase revealed PKC1B promoter activity exclusively in sensory neurons and interneurons. Immunofluorescence microscopy disclosed that the PKC1B polypeptide is located in the processes (axons and dendrites) and perinuclear regions of ∼ 75 neurons that constitute the sensory circuitry of the nematode. The intracellular localization of PKC1B and the enzyme's differential solubility in ionic and nonionic detergents suggest that the kinase is associated with membranes and the cytoskeleton.",
author = "Marianne Land and Alma Islas-Trejo and Freedman, {Jonathan H.} and Rubin, {Charles S.}",
year = "1994",
month = "3",
day = "25",
language = "English (US)",
volume = "269",
pages = "9234--9244",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "12",

}

TY - JOUR

T1 - Structure and expression of a novel, neuronal protein kinase C (PKC1B) from Caenorhabditis elegans

T2 - PKC1B is expressed selectively in neurons that receive, transmit, and process environmental signals

AU - Land, Marianne

AU - Islas-Trejo, Alma

AU - Freedman, Jonathan H.

AU - Rubin, Charles S.

PY - 1994/3/25

Y1 - 1994/3/25

N2 - The nematode Caenorhabditis elegans provides an advantageous system for investigating the regulation, expression, and functions of protein kinase C (PKC) isoforms. We cloned and characterized cDNAs encoding a novel C. elegans PKC designated PKC1B. The predicted PKC1B polypeptide contains features characteristic of the nPKC subfamily of PKC isoforms. The levels of PKC1B and its cognate mRNA vary over a 7-fold range during C. elegans postembryonic development. PKC1B protein and mRNA are abundant at the earliest larval stage, but their relative concentrations decrease coordinately in late larvae. Embryos, which are enriched in PKC1B mRNA, contain little PKC1B protein. Thus, PKC1B expression is regulated at a translational or post-translational level during early development. Cells engaged in PKC1B gene transcription were identified in transgenic C. elegans that carry the lacZ gene under the regulation of the PKC1B promoter. Staining for β-galactosidase revealed PKC1B promoter activity exclusively in sensory neurons and interneurons. Immunofluorescence microscopy disclosed that the PKC1B polypeptide is located in the processes (axons and dendrites) and perinuclear regions of ∼ 75 neurons that constitute the sensory circuitry of the nematode. The intracellular localization of PKC1B and the enzyme's differential solubility in ionic and nonionic detergents suggest that the kinase is associated with membranes and the cytoskeleton.

AB - The nematode Caenorhabditis elegans provides an advantageous system for investigating the regulation, expression, and functions of protein kinase C (PKC) isoforms. We cloned and characterized cDNAs encoding a novel C. elegans PKC designated PKC1B. The predicted PKC1B polypeptide contains features characteristic of the nPKC subfamily of PKC isoforms. The levels of PKC1B and its cognate mRNA vary over a 7-fold range during C. elegans postembryonic development. PKC1B protein and mRNA are abundant at the earliest larval stage, but their relative concentrations decrease coordinately in late larvae. Embryos, which are enriched in PKC1B mRNA, contain little PKC1B protein. Thus, PKC1B expression is regulated at a translational or post-translational level during early development. Cells engaged in PKC1B gene transcription were identified in transgenic C. elegans that carry the lacZ gene under the regulation of the PKC1B promoter. Staining for β-galactosidase revealed PKC1B promoter activity exclusively in sensory neurons and interneurons. Immunofluorescence microscopy disclosed that the PKC1B polypeptide is located in the processes (axons and dendrites) and perinuclear regions of ∼ 75 neurons that constitute the sensory circuitry of the nematode. The intracellular localization of PKC1B and the enzyme's differential solubility in ionic and nonionic detergents suggest that the kinase is associated with membranes and the cytoskeleton.

UR - http://www.scopus.com/inward/record.url?scp=0028240128&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028240128&partnerID=8YFLogxK

M3 - Article

C2 - 8132661

AN - SCOPUS:0028240128

VL - 269

SP - 9234

EP - 9244

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 12

ER -