Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers

Tingting Wang, Guoxiang Cai, Yunping Qiu, Na Fei, Menghui Zhang, Xiaoyan Pang, Wei Jia, Sanjun Cai, Liping Zhao

Research output: Contribution to journalArticle

429 Citations (Scopus)

Abstract

Despite a long-suspected role in the development of human colorectal cancer (CRC), the composition of gut microbiota in CRC patients has not been adequately described. In this study, fecal bacterial diversity in CRC patients (n=46) and healthy volunteers (n=56) were profiled by 454 pyrosequencing of the V3 region of the 16S ribosomal RNA gene. Both principal component analysis and UniFrac analysis showed structural segregation between the two populations. Forty-eight operational taxonomic units (OTUs) were identified by redundancy analysis as key variables significantly associated with the structural difference. One OTU closely related to Bacteroides fragilis was enriched in the gut microbiota of CRC patients, whereas three OTUs related to Bacteroides vulgatus and Bacteroides uniformis were enriched in that of healthy volunteers. A total of 11 OTUs belonging to the genera Enterococcus, Escherichia/Shigella, Klebsiella, Streptococcus and Peptostreptococcus were significantly more abundant in the gut microbiota of CRC patients, and 5 OTUs belonging to the genus Roseburia and other butyrate-producing bacteria of the family Lachnospiraceae were less abundant. Real-time quantitative PCR further validated the significant reduction of butyrate-producing bacteria in the gut microbiota of CRC patients by measuring the copy numbers of butyryl-coenzyme A CoA transferase genes (Mann-Whitney test, P<0.01). Reduction of butyrate producers and increase of opportunistic pathogens may constitute a major structural imbalance of gut microbiota in CRC patients.

Original languageEnglish (US)
Pages (from-to)320-329
Number of pages10
JournalISME Journal
Volume6
Issue number2
DOIs
StatePublished - Feb 2012
Externally publishedYes

Fingerprint

colorectal neoplasms
intestinal microorganisms
volunteers
Colorectal Neoplasms
cancer
Healthy Volunteers
Butyrates
butyrates
Coenzyme A-Transferases
Bacteroides
Bacteroides uniformis
Roseburia
Bacteroides vulgatus
CoA-transferases
Peptostreptococcus
Bacteroides fragilis
16S Ribosomal RNA
Bacteria
Escherichia
Shigella

Keywords

  • colorectal cancer
  • gut microbiota
  • pyrosequencing

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

Cite this

Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers. / Wang, Tingting; Cai, Guoxiang; Qiu, Yunping; Fei, Na; Zhang, Menghui; Pang, Xiaoyan; Jia, Wei; Cai, Sanjun; Zhao, Liping.

In: ISME Journal, Vol. 6, No. 2, 02.2012, p. 320-329.

Research output: Contribution to journalArticle

Wang, T, Cai, G, Qiu, Y, Fei, N, Zhang, M, Pang, X, Jia, W, Cai, S & Zhao, L 2012, 'Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers', ISME Journal, vol. 6, no. 2, pp. 320-329. https://doi.org/10.1038/ismej.2011.109
Wang, Tingting ; Cai, Guoxiang ; Qiu, Yunping ; Fei, Na ; Zhang, Menghui ; Pang, Xiaoyan ; Jia, Wei ; Cai, Sanjun ; Zhao, Liping. / Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers. In: ISME Journal. 2012 ; Vol. 6, No. 2. pp. 320-329.
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