@article{8a4c3ff59ab84414a188307fb17a89b7,
title = "Structural basis for RNA polymerase III transcription repression by Maf1",
abstract = "Maf1 is a conserved inhibitor of RNA polymerase III (Pol III) that influences phenotypes ranging from metabolic efficiency to lifespan. Here, we present a 3.3-{\AA}-resolution cryo-EM structure of yeast Maf1 bound to Pol III, establishing that Maf1 sequesters Pol III elements involved in transcription initiation and binds the mobile C34 winged helix 2 domain, sealing off the active site. The Maf1 binding site overlaps with that of TFIIIB in the preinitiation complex.",
author = "Vorl{\"a}nder, {Matthias K.} and Florence Baudin and Moir, {Robyn D.} and Ren{\'e} Wetzel and Hagen, {Wim J.H.} and Willis, {Ian M.} and M{\"u}ller, {Christoph W.}",
note = "Funding Information: This work was supported by an ERC Advanced Grant (ERC-2013-AdG340964-POL1PIC) to C.W.M. and R.W., a National Institutes of Health Grant (GM120358) to I.M.W. and an EMBL International PhD program award to M.K.V. We thank M. Girbig for help with transcription assays and critical reading of the manuscript and F. Weis for EM support. We are grateful to T. Hoffmann and J. Pecar for maintaining the high performance computing environment for EM data processing at EMBL.",
year = "2020",
month = mar,
day = "1",
doi = "10.1038/s41594-020-0383-y",
language = "English (US)",
volume = "27",
pages = "229--232",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "Nature Publishing Group",
number = "3",
}