Structural and Electrostatic Properties of the 5-HT3 Receptor Pore Revealed by Substituted Cysteine Accessibility Mutagenesis

David C. Reeves, Eric N. Goren, Myles H. Akabas, Sarah C.R. Lummis

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


5-HT3 receptors are members of the Cys loop family of ligand-gated ion channels. We used the substituted cysteine accessibility method to identify amino acid residues in the channel forming domain, M2 that face the water-accessible surface and to locate their position in the ion conduction pathway. Cysteine was substituted for each residue, one at a time, in the M2 segment (Asp274-Asp298). 5-Hydroxytryptamine EC50 values for functional mutants did not vary from wild type (1.4 ± 0.2 μM) by more than 10-fold, and five mutants were nonfunctional. Covalent modification of the mutant receptors with sulfydryl reagents revealed 11 residues to be water-accessible, with a pattern consistent with an α-helix except at Leu285 and Leu293. The data suggest that charge selectivity begins at a more cytoplasmic level than Val 291. Modification at some positions (Val291, Leu 293, Ile294, Leu287 and Ser280) resulted in channels that were locked open. Reaction rates with accessible cysteines were voltage-dependent at some residues, suggesting that access occurs via the ion channel. Overall the data observed are similar but not identical to that reported for other members of the family and confirms the high degree of structural and functional homology between receptors in the Cys loop receptor family.

Original languageEnglish (US)
Pages (from-to)42035-42042
Number of pages8
JournalJournal of Biological Chemistry
Issue number45
StatePublished - Nov 9 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Structural and Electrostatic Properties of the 5-HT<sub>3</sub> Receptor Pore Revealed by Substituted Cysteine Accessibility Mutagenesis'. Together they form a unique fingerprint.

Cite this