Structural analysis of gelsolin using synchrotron protein footprinting.

Janna G. Kiselar, Paul A. Janmey, Steven C. Almo, Mark R. Chance

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Protein footprinting provides detailed structural information on protein structure in solution by directly identifying accessible and hydroxyl radical-reactive side chain residues. Radiolytic generation of hydroxyl radicals using millisecond pulses of a synchrotron "white" beam results in the formation of stable side chain oxidation products, which can be digested with proteases for mass spectrometry (MS) analysis. Liquid chromatography-coupled MS and tandem MS methods allow for the quantitation of the ratio of modified and unmodified peptides and identify the specific side chain probes that are oxidized, respectively. The ability to monitor the changes in accessibility of multiple side chain probes by monitoring increases or decreases in their oxidation rates as a function of ligand binding provides an efficient and powerful tool for analyzing protein structure and dynamics. In this study, we probe the detailed structural features of gelsolin in its "inactive" and Ca2+-activated state. Oxidation rate data for 81 peptides derived from the trypsin digestion of gelsolin are presented; 60 of these peptides were observed not to be oxidized, and 21 had detectable oxidation rates. We also report the Ca2+-dependent changes in oxidation for all 81 peptides. Fifty-nine remained unoxidized, five increased their oxidation rate, and two experienced protections. Tandem mass spectrometry was used to identify the specific side chain probes responsible for the Ca2+-insensitive and Ca2+-dependent responses. These data are consistent with crystallographic data for the inactive form of gelsolin in terms of the surface accessibility of reactive residues within the protein. The results demonstrate that radiolytic protein footprinting can provide detailed structural information on the conformational dynamics of ligand-induced structural changes, and the data provide a detailed model for gelsolin activation.

Original languageEnglish (US)
Pages (from-to)1120-1132
Number of pages13
JournalMolecular & cellular proteomics : MCP.
Volume2
Issue number10
StatePublished - Oct 2003

Fingerprint

Protein Footprinting
Gelsolin
Synchrotrons
Structural analysis
Tandem Mass Spectrometry
Oxidation
Peptides
Mass spectrometry
Hydroxyl Radical
Proteins
Ligands
Liquid Chromatography
Trypsin
Digestion
Mass Spectrometry
Peptide Hydrolases
Liquid chromatography
Chemical activation
Monitoring

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structural analysis of gelsolin using synchrotron protein footprinting. / Kiselar, Janna G.; Janmey, Paul A.; Almo, Steven C.; Chance, Mark R.

In: Molecular & cellular proteomics : MCP., Vol. 2, No. 10, 10.2003, p. 1120-1132.

Research output: Contribution to journalArticle

Kiselar, Janna G. ; Janmey, Paul A. ; Almo, Steven C. ; Chance, Mark R. / Structural analysis of gelsolin using synchrotron protein footprinting. In: Molecular & cellular proteomics : MCP. 2003 ; Vol. 2, No. 10. pp. 1120-1132.
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