Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis

Junmin Wang; Beilei Ge; Zihong Li; Fangxia Guan; Feifei Li

doi:10.1016/j.carbpol.2015.12.050

Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis

Junmin Wang, Beilei Ge, Zihong Li, Fangxia Guan, Feifei Li

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Four fractional polysaccharides (CAPS₃₀, CAPS₅₀, CAPS₇₀ and CAPS₈₀) and total polysaccharides (CAPS_t) were obtained from Angelica sinensis. Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS). Their immunoregulation activities were further compared in vitro. The results showed that they displayed different structural features and immuno-enhancement activities. They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes. Further experiments showed that CAPS₅₀ and CAPS₇₀ could increase the ratio of CD3⁺CD56⁺ cells to some extent. These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS₇₀, with the molecular weights of 20.82 kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.

Original language	English (US)
Pages (from-to)	6-12
Number of pages	7
Journal	Carbohydrate Polymers
Volume	140
DOIs	https://doi.org/10.1016/j.carbpol.2015.12.050
State	Published - Apr 20 2016
Externally published	Yes

Keywords

Angelica sinensis polysaccharides
Immunoregulation
Structural analysis

ASJC Scopus subject areas

Polymers and Plastics
Organic Chemistry
Materials Chemistry

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title = "Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis",

abstract = "Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis. Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS). Their immunoregulation activities were further compared in vitro. The results showed that they displayed different structural features and immuno-enhancement activities. They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes. Further experiments showed that CAPS50 and CAPS70 could increase the ratio of CD3+CD56+ cells to some extent. These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82 kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.",

keywords = "Angelica sinensis polysaccharides, Immunoregulation, Structural analysis",

author = "Junmin Wang and Beilei Ge and Zihong Li and Fangxia Guan and Feifei Li",

year = "2016",

month = apr,

day = "20",

doi = "10.1016/j.carbpol.2015.12.050",

language = "English (US)",

volume = "140",

pages = "6--12",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

publisher = "Elsevier Limited",

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TY - JOUR

T1 - Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis

AU - Wang, Junmin

AU - Ge, Beilei

AU - Li, Zihong

AU - Guan, Fangxia

AU - Li, Feifei

PY - 2016/4/20

Y1 - 2016/4/20

N2 - Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis. Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS). Their immunoregulation activities were further compared in vitro. The results showed that they displayed different structural features and immuno-enhancement activities. They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes. Further experiments showed that CAPS50 and CAPS70 could increase the ratio of CD3+CD56+ cells to some extent. These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82 kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.

AB - Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis. Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS). Their immunoregulation activities were further compared in vitro. The results showed that they displayed different structural features and immuno-enhancement activities. They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes. Further experiments showed that CAPS50 and CAPS70 could increase the ratio of CD3+CD56+ cells to some extent. These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82 kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.

KW - Angelica sinensis polysaccharides

KW - Immunoregulation

KW - Structural analysis

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