Stoichiometry of DNA strand scission and aldehyde formation by bleomycin

R. M. Burger, J. Peisach, Susan Band Horwitz

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

A colorimetric assay of DNA breakage by bleomycin has been standardized and indicates that strand scission is stoichiometric with the formation of a single equivalent of an aldehydic compound consisting of base plus deoxyribose carbons 1' to 3'. Both strand scission and aldehyde formation require the presence of O2. An alternate DNA lesion inflicted by bleomycin, alkali labilization, is O2-independent, as is the accompanying release of free bases.

Original languageEnglish (US)
Pages (from-to)8612-8614
Number of pages3
JournalJournal of Biological Chemistry
Volume257
Issue number15
StatePublished - 1982

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Bleomycin
Aldehydes
Stoichiometry
Deoxyribose
DNA
Alkalies
Assays
Carbon

ASJC Scopus subject areas

  • Biochemistry

Cite this

Stoichiometry of DNA strand scission and aldehyde formation by bleomycin. / Burger, R. M.; Peisach, J.; Band Horwitz, Susan.

In: Journal of Biological Chemistry, Vol. 257, No. 15, 1982, p. 8612-8614.

Research output: Contribution to journalArticle

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