Stimulatory effect of vitamin C on autophagy in glial cells

Antonio Martin, James A. Joseph, Ana Maria Cuervo

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Intracellular accumulation of damaged or abnormal proteins is a common event associated with numerous neurodegenerative diseases and other age-related pathologies. Increasing the activity of the intracellular proteolytic systems normally responsible for the removal of these abnormal proteins might be beneficial in lessening the severity or development of those pathologies. In this study we have used human astrocyte glial cells to investigate the effect of vitamin C (ascorbate) on the intracellular turnover of proteins. Supplementation of the culture medium with physiological concentrations of vitamin C did not affect protein synthesis, but did increase the rate of protein degradation by lysosomes. Vitamin C accelerated the degradation of intra- and extracellular proteins targeted to the lysosomal lumen by autophagic and heterophagic pathways. At the doses analyzed, vitamin C lowered and stabilized the acidic intralysosomal pH at values that result in maximum activation of the lysosomal hydrolases.

Original languageEnglish (US)
Pages (from-to)538-549
Number of pages12
JournalJournal of Neurochemistry
Volume82
Issue number3
DOIs
StatePublished - 2002

Fingerprint

Autophagy
Neuroglia
Ascorbic Acid
Proteins
Pathology
Neurodegenerative diseases
Hydrolases
Degradation
Lysosomes
Astrocytes
Neurodegenerative Diseases
Proteolysis
Culture Media
Chemical activation

Keywords

  • Ascorbic acid
  • Astrocytes
  • Cathepsins
  • Lysosomes
  • Protein degradation
  • Vitamins

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Stimulatory effect of vitamin C on autophagy in glial cells. / Martin, Antonio; Joseph, James A.; Cuervo, Ana Maria.

In: Journal of Neurochemistry, Vol. 82, No. 3, 2002, p. 538-549.

Research output: Contribution to journalArticle

Martin, Antonio ; Joseph, James A. ; Cuervo, Ana Maria. / Stimulatory effect of vitamin C on autophagy in glial cells. In: Journal of Neurochemistry. 2002 ; Vol. 82, No. 3. pp. 538-549.
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