Stimulation of IgE and IgA production by CD45RA T helper cells in atopic patients

Solveig Nasert, Nina Burtchen, Fatimah Kussebi, Martina Millner, Richard Kroczek, Thomas Jung, Reinhard Schwinzer, Ulrich Wahn, Harald Renz

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The role of CD45RA T cells on allergen-dependent lymphocyte functions was analyzed in atopic patients. As compared with age-matched nonatopic controls, atopic patients exhibited a significantly (p < 0.01) increased frequency of CD45RA T cells in peripheral blood. Concentration of serum IgE correlated with increases in this T cell subset. In contrast to nonatopic controls, not only CD45RO but also CD45RA T cells from atopic patients provide help for allergen-stimulated IgE and IgA production, and they act in a synergistic fashion. Transwell coculture experiments revealed that optimal production of IgE and IgA required physical contact of CD45RA T cells with B cells. Freshly prepared and in vitro-activated CD45RO and CD45RA T cells from atopic patients showed an increased expression of CD40 ligand when compared with nonatopic individuals. In addition, CD45RA (and CD45RO) T cells from atopic individuals produced IL-4, IL-5, and IFN-γ when stimulated with mitogens. Whereas stimulation of normal lymphocytes with tetanus Ag was followed by conversion of the CD45RA to the CD45RO phenotype, T cells from atopic donors did not acquire the CD45RO isoform to the same degree despite T cell activation. In atopic patients, addition of IL-4 to anti-CD3/anti-TCR stimulated CD45RA T cell prevented the shift towards the CD45RO phenotype. These data indicate that a subset of CD45RA T cells plays a unique role as effector T cells regulating IgE and IgA production in atopic patients.

Original languageEnglish (US)
Pages (from-to)441-448
Number of pages8
JournalJournal of Immunology
Volume157
Issue number1
StatePublished - Jul 1 1996

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Nasert, S., Burtchen, N., Kussebi, F., Millner, M., Kroczek, R., Jung, T., Schwinzer, R., Wahn, U., & Renz, H. (1996). Stimulation of IgE and IgA production by CD45RA T helper cells in atopic patients. Journal of Immunology, 157(1), 441-448.