Stimulation of IgE and IgA production by CD45RA T helper cells in atopic patients

Solveig Nasert, Nina Burtchen, Fatimah Kussebi, Martina Millner, Richard Kroczek, Thomas Jung, Reinhard Schwinzer, Ulrich Wahn, Harald Renz

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The role of CD45RA T cells on allergen-dependent lymphocyte functions was analyzed in atopic patients. As compared with age-matched nonatopic controls, atopic patients exhibited a significantly (p < 0.01) increased frequency of CD45RA T cells in peripheral blood. Concentration of serum IgE correlated with increases in this T cell subset. In contrast to nonatopic controls, not only CD45RO but also CD45RA T cells from atopic patients provide help for allergen-stimulated IgE and IgA production, and they act in a synergistic fashion. Transwell coculture experiments revealed that optimal production of IgE and IgA required physical contact of CD45RA T cells with B cells. Freshly prepared and in vitro-activated CD45RO and CD45RA T cells from atopic patients showed an increased expression of CD40 ligand when compared with nonatopic individuals. In addition, CD45RA (and CD45RO) T cells from atopic individuals produced IL-4, IL-5, and IFN-γ when stimulated with mitogens. Whereas stimulation of normal lymphocytes with tetanus Ag was followed by conversion of the CD45RA to the CD45RO phenotype, T cells from atopic donors did not acquire the CD45RO isoform to the same degree despite T cell activation. In atopic patients, addition of IL-4 to anti-CD3/anti-TCR stimulated CD45RA T cell prevented the shift towards the CD45RO phenotype. These data indicate that a subset of CD45RA T cells plays a unique role as effector T cells regulating IgE and IgA production in atopic patients.

Original languageEnglish (US)
Pages (from-to)441-448
Number of pages8
JournalJournal of Immunology
Volume157
Issue number1
StatePublished - Jul 1 1996
Externally publishedYes

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Helper-Inducer T-Lymphocytes
Immunoglobulin A
Immunoglobulin E
T-Lymphocytes
T-Lymphocyte Subsets
Interleukin-4
Allergens
Phenotype
CD40 Ligand
Interleukin-5
Tetanus
Lymphocyte Activation
Coculture Techniques
Mitogens
Protein Isoforms
B-Lymphocytes
Tissue Donors
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Nasert, S., Burtchen, N., Kussebi, F., Millner, M., Kroczek, R., Jung, T., ... Renz, H. (1996). Stimulation of IgE and IgA production by CD45RA T helper cells in atopic patients. Journal of Immunology, 157(1), 441-448.

Stimulation of IgE and IgA production by CD45RA T helper cells in atopic patients. / Nasert, Solveig; Burtchen, Nina; Kussebi, Fatimah; Millner, Martina; Kroczek, Richard; Jung, Thomas; Schwinzer, Reinhard; Wahn, Ulrich; Renz, Harald.

In: Journal of Immunology, Vol. 157, No. 1, 01.07.1996, p. 441-448.

Research output: Contribution to journalArticle

Nasert, S, Burtchen, N, Kussebi, F, Millner, M, Kroczek, R, Jung, T, Schwinzer, R, Wahn, U & Renz, H 1996, 'Stimulation of IgE and IgA production by CD45RA T helper cells in atopic patients', Journal of Immunology, vol. 157, no. 1, pp. 441-448.
Nasert S, Burtchen N, Kussebi F, Millner M, Kroczek R, Jung T et al. Stimulation of IgE and IgA production by CD45RA T helper cells in atopic patients. Journal of Immunology. 1996 Jul 1;157(1):441-448.
Nasert, Solveig ; Burtchen, Nina ; Kussebi, Fatimah ; Millner, Martina ; Kroczek, Richard ; Jung, Thomas ; Schwinzer, Reinhard ; Wahn, Ulrich ; Renz, Harald. / Stimulation of IgE and IgA production by CD45RA T helper cells in atopic patients. In: Journal of Immunology. 1996 ; Vol. 157, No. 1. pp. 441-448.
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