Stimulation by cAMP of erythropoietin secretion by an established human renal carcinoma cell line

J. B. Sherwood, Edward R. Burns, D. Shouval

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We used our recently reported stable, transformed human renal carcinoma cell line as a model system to study the role of 3',5'-adenosine monophosphate (cAMP) in erythropoietin secretion. The erythropoietin produced by these cells is both biologically active and immunologically cross-reactive with purified native human hormone in our radioimmunoassay. Erythropoietin release by these renal carcinoma cells appears to be stimulated by cAMP as well as by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (MIX). The response to cAMP involves a rapid and enhanced release of hormone, which occurred within 30 minutes of exposure of the cells to the effector and continued for at least 4 hours. Intracellular erythropoietin was higher in the control cultures than in the cells treated with cAMP, suggesting that cAMP stimulates the release of a storage pool of hormone. The ability of cAMP and MIX to elicit the release of erythropoietin suggests that a cAMP-mediated mechanism is involved in the release of this hormone.

Original languageEnglish (US)
Pages (from-to)1053-1057
Number of pages5
JournalBlood
Volume69
Issue number4
StatePublished - 1987

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Erythropoietin
Cells
Carcinoma
Kidney
Cell Line
Hormones
1-Methyl-3-isobutylxanthine
Phosphodiesterase Inhibitors
Adenosine Monophosphate
Population Groups
Renal Cell Carcinoma
Radioimmunoassay
Cell Culture Techniques

ASJC Scopus subject areas

  • Hematology

Cite this

Stimulation by cAMP of erythropoietin secretion by an established human renal carcinoma cell line. / Sherwood, J. B.; Burns, Edward R.; Shouval, D.

In: Blood, Vol. 69, No. 4, 1987, p. 1053-1057.

Research output: Contribution to journalArticle

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