Sterilization of Mycobacterium tuberculosis Erdman samples by antimicrobial fixation in a biosafety level 3 laboratory

J. R. Schwebach, Jr Jacobs, A. Casadevall

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Abstract

Incomplete sterilization of Mycobacterium tuberculosis Erdman cultures followed 1 h of incubation in low concentrations of glutaraldehyde (0.5 and 1.0%) or azide. In contrast, 2.5% glutaraldehyde, paraformaldehyde (2 or 4%), Vesphine IIse or 5% formalin sterilized these samples after 1 h. These results suggest caution in removing fixed M. tuberculosis samples from biosafety level 3.

Original languageEnglish (US)
Pages (from-to)769-771
Number of pages3
JournalJournal of Clinical Microbiology
Volume39
Issue number2
DOIs
Publication statusPublished - Feb 24 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology (medical)

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