TY - JOUR
T1 - Steric course of the hydrolysis of α,α-trehalose and α-d-glucosyl fluoride catalyzed by pig kidney trehalase
AU - Nakano, Masatoshi
AU - Brewer, Curtis F.
AU - Kasumi, Takafumi
AU - Hehre, Edward J.
PY - 1989/12/1
Y1 - 1989/12/1
N2 - We are unable to confirm the report of Labat et al. that pig kidney trehalase hydrolyzes α,α-trehalose to form solely α-d-glucose. Highly purified trehalase from pig renal cortex was found, in reactions monitored by 1H-n.m.r. spectra, to hydrolyze α,α-trehalose with the formation of both α- and β-d-glucose. That the β anomer constitutes the enzymically mobilized glucosyl residue is indicated by the further finding that β-d-glucose is the product formed on hydrolysis of α-d-glucosyl fluoride by the enzyme. Present results show the stereochemical behavior of pig kidney trehalase in hydrolyzing α,α-trehalose to be indistinguishable from that reported by ourselves and others for trehalase preparations from a range of biological sources including rabbit renal cortex.
AB - We are unable to confirm the report of Labat et al. that pig kidney trehalase hydrolyzes α,α-trehalose to form solely α-d-glucose. Highly purified trehalase from pig renal cortex was found, in reactions monitored by 1H-n.m.r. spectra, to hydrolyze α,α-trehalose with the formation of both α- and β-d-glucose. That the β anomer constitutes the enzymically mobilized glucosyl residue is indicated by the further finding that β-d-glucose is the product formed on hydrolysis of α-d-glucosyl fluoride by the enzyme. Present results show the stereochemical behavior of pig kidney trehalase in hydrolyzing α,α-trehalose to be indistinguishable from that reported by ourselves and others for trehalase preparations from a range of biological sources including rabbit renal cortex.
UR - http://www.scopus.com/inward/record.url?scp=0024793937&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024793937&partnerID=8YFLogxK
U2 - 10.1016/0008-6215(89)85013-X
DO - 10.1016/0008-6215(89)85013-X
M3 - Article
C2 - 2620297
AN - SCOPUS:0024793937
SN - 0008-6215
VL - 194
SP - 139
EP - 144
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - C
ER -