Steric course of the hydrolysis of α,α-trehalose and α-d-glucosyl fluoride catalyzed by pig kidney trehalase

Masatoshi Nakano, Curtis F. Brewer, Takafumi Kasumi, Edward J. Hehre

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We are unable to confirm the report of Labat et al. that pig kidney trehalase hydrolyzes α,α-trehalose to form solely α-d-glucose. Highly purified trehalase from pig renal cortex was found, in reactions monitored by 1H-n.m.r. spectra, to hydrolyze α,α-trehalose with the formation of both α- and β-d-glucose. That the β anomer constitutes the enzymically mobilized glucosyl residue is indicated by the further finding that β-d-glucose is the product formed on hydrolysis of α-d-glucosyl fluoride by the enzyme. Present results show the stereochemical behavior of pig kidney trehalase in hydrolyzing α,α-trehalose to be indistinguishable from that reported by ourselves and others for trehalase preparations from a range of biological sources including rabbit renal cortex.

Original languageEnglish (US)
Pages (from-to)139-144
Number of pages6
JournalCarbohydrate Research
Issue numberC
Publication statusPublished - Dec 1 1989


ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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