Stereoselectivity of Isolated Dehydratase Domains of the Borrelidin Polyketide Synthase

Implications for cis Double Bond Formation

Olivia M. Vergnolle, Frank Hahn, Abel Baerga-Ortiz, Peter F. Leadlay, Jennifer N. Andexer

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Not a matter of preference: The polyketide borrelidin contains both a trans and a cis double bond. The corresponding dehydratases in the polyketide synthase are found to have identical preferences for thioester substrates in vitro and to generate trans double bonds, undermining the idea that cis double bonds arise from dehydration of 3S rather than 3R alcohols.

Original languageEnglish (US)
Pages (from-to)1011-1014
Number of pages4
JournalChemBioChem
Volume12
Issue number7
DOIs
StatePublished - May 2 2011
Externally publishedYes

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Polyketide Synthases
Hydro-Lyases
Polyketides
Stereoselectivity
Dehydration
Alcohols
Substrates
borrelidin
In Vitro Techniques

Keywords

  • Borrelidin
  • Dehydration
  • Double bonds
  • Enzyme catalysis
  • Polyketides

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology

Cite this

Stereoselectivity of Isolated Dehydratase Domains of the Borrelidin Polyketide Synthase : Implications for cis Double Bond Formation. / Vergnolle, Olivia M.; Hahn, Frank; Baerga-Ortiz, Abel; Leadlay, Peter F.; Andexer, Jennifer N.

In: ChemBioChem, Vol. 12, No. 7, 02.05.2011, p. 1011-1014.

Research output: Contribution to journalArticle

Vergnolle, Olivia M. ; Hahn, Frank ; Baerga-Ortiz, Abel ; Leadlay, Peter F. ; Andexer, Jennifer N. / Stereoselectivity of Isolated Dehydratase Domains of the Borrelidin Polyketide Synthase : Implications for cis Double Bond Formation. In: ChemBioChem. 2011 ; Vol. 12, No. 7. pp. 1011-1014.
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