Stereochemistry of the Fe(II)- and Fe(III)-cyanide complexes of the homodimeric Scapharca inaequivalvis hemoglobin. A resonance Raman and FTIR study

Alberto Boffi, Emilia Chiancone, Satoshi Takahashi, Denis L. Rousseau

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Abstract

Resonance Raman measurements carried out in parallel on ferrous and ferric Scapharca inaequivalvis dimeric hemoglobin cyanide derivatives allowed the identification of the electron density marker bands and heme core size marker bands in both derivatives in comparison with those obtained for the carbonmonoxy and deoxy adducts. The iron cyanide stretching mode, measured for the first time in a ferrous hemoprotein, has been detected at 455 cm-1, only 6 cm-1 lower than in the corresponding ferric derivative. This finding demonstrates that the large free energy difference for complex formation between the two derivatives is not concentrated on the Fe-C bond. The internal stretching frequencies of the ligand in the ferric and ferrous derivatives have been identified by FTIR and Raman measurements using different cyanide isotopes. The frequency decreases in the order Fe(III)-CN adduct, free cyanide, Fe(II)-CN adduct, consistent with the behavior observed in inorganic complexes and horseradish peroxidase. The main feature emerging from these data is that cyanide, at variance with oxygen and carbon monoxide, binds to ferrous iron with only a minor perturbation of the electronic structure of the heine. The functional counterpart of this effect is the absence of cooperative cyanide binding in HbI.

Original languageEnglish (US)
Pages (from-to)4505-4509
Number of pages5
JournalBiochemistry
Volume36
Issue number15
DOIs
StatePublished - Apr 15 1997

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Scapharca
Stereochemistry
Cyanides
Fourier Transform Infrared Spectroscopy
Hemoglobins
Derivatives
Stretching
Iron
Horseradish Peroxidase
Carbon Monoxide
Heme
Isotopes
Free energy
Electronic structure
Carrier concentration
Electrons
Oxygen
Ligands

ASJC Scopus subject areas

  • Biochemistry

Cite this

Stereochemistry of the Fe(II)- and Fe(III)-cyanide complexes of the homodimeric Scapharca inaequivalvis hemoglobin. A resonance Raman and FTIR study. / Boffi, Alberto; Chiancone, Emilia; Takahashi, Satoshi; Rousseau, Denis L.

In: Biochemistry, Vol. 36, No. 15, 15.04.1997, p. 4505-4509.

Research output: Contribution to journalArticle

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abstract = "Resonance Raman measurements carried out in parallel on ferrous and ferric Scapharca inaequivalvis dimeric hemoglobin cyanide derivatives allowed the identification of the electron density marker bands and heme core size marker bands in both derivatives in comparison with those obtained for the carbonmonoxy and deoxy adducts. The iron cyanide stretching mode, measured for the first time in a ferrous hemoprotein, has been detected at 455 cm-1, only 6 cm-1 lower than in the corresponding ferric derivative. This finding demonstrates that the large free energy difference for complex formation between the two derivatives is not concentrated on the Fe-C bond. The internal stretching frequencies of the ligand in the ferric and ferrous derivatives have been identified by FTIR and Raman measurements using different cyanide isotopes. The frequency decreases in the order Fe(III)-CN adduct, free cyanide, Fe(II)-CN adduct, consistent with the behavior observed in inorganic complexes and horseradish peroxidase. The main feature emerging from these data is that cyanide, at variance with oxygen and carbon monoxide, binds to ferrous iron with only a minor perturbation of the electronic structure of the heine. The functional counterpart of this effect is the absence of cooperative cyanide binding in HbI.",
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AU - Rousseau, Denis L.

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AB - Resonance Raman measurements carried out in parallel on ferrous and ferric Scapharca inaequivalvis dimeric hemoglobin cyanide derivatives allowed the identification of the electron density marker bands and heme core size marker bands in both derivatives in comparison with those obtained for the carbonmonoxy and deoxy adducts. The iron cyanide stretching mode, measured for the first time in a ferrous hemoprotein, has been detected at 455 cm-1, only 6 cm-1 lower than in the corresponding ferric derivative. This finding demonstrates that the large free energy difference for complex formation between the two derivatives is not concentrated on the Fe-C bond. The internal stretching frequencies of the ligand in the ferric and ferrous derivatives have been identified by FTIR and Raman measurements using different cyanide isotopes. The frequency decreases in the order Fe(III)-CN adduct, free cyanide, Fe(II)-CN adduct, consistent with the behavior observed in inorganic complexes and horseradish peroxidase. The main feature emerging from these data is that cyanide, at variance with oxygen and carbon monoxide, binds to ferrous iron with only a minor perturbation of the electronic structure of the heine. The functional counterpart of this effect is the absence of cooperative cyanide binding in HbI.

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