Stepwise assembly of fibrin bilayers on self-assembled monolayers of alkanethiolates: Influence of surface chemistry

Hua Wang, Buddy D. Ratner, E. Helene Sage, Shaoyi Jiang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Fibrin bilayers are formed in a stepwise manner on four model surfaces: hydrophobic, neutral hydrophilic, positively charged, and negatively charged. These surfaces are self-assembled monolayers (SAMs) of alkanethiolates on gold with different terminal groups. A surface plasmon resonance (SPR) sensor is used to monitor the formation of fibrin bilayers in real time. The structures of the fibrin bilayers formed on SAMs of different surface chemistries are proposed, based on the amount of fibrinogen in each layer. The efficiency of the physically adsorbed fibrinogen to promote fibrin formation is assessed by the ratio of the amount of fibrinogen in the second layer to that in the first layer. It is shown for the first time that the orientation/conformation of an adsorbed fibrinogen layer determined by underlying surface chemistry affects its reactivity with soluble fibrinogen, resulting in fibrin bilayers with different molecular arrangements. Bovine aortic endothelial cells cultured on fibrin bilayers formed on CH 3- and OH- SAMs exhibited different morphologies and growth characteristics. Results show that fibrin bilayers with different molecular arrangements and thus different mechanochemical properties lead to different cellular responses.

Original languageEnglish (US)
Pages (from-to)8504-8508
Number of pages5
JournalJournal of Physical Chemistry C
Volume111
Issue number24
DOIs
StatePublished - Jun 21 2007
Externally publishedYes

Fingerprint

fibrin
Self assembled monolayers
Surface chemistry
Fibrin
fibrinogen
assembly
chemistry
Fibrinogen
Endothelial cells
Surface plasmon resonance
Crystal orientation
Conformations
Gold
Sensors
surface plasmon resonance
cultured cells
reactivity
methylidyne
gold
sensors

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Energy(all)

Cite this

Stepwise assembly of fibrin bilayers on self-assembled monolayers of alkanethiolates : Influence of surface chemistry. / Wang, Hua; Ratner, Buddy D.; Sage, E. Helene; Jiang, Shaoyi.

In: Journal of Physical Chemistry C, Vol. 111, No. 24, 21.06.2007, p. 8504-8508.

Research output: Contribution to journalArticle

Wang, Hua ; Ratner, Buddy D. ; Sage, E. Helene ; Jiang, Shaoyi. / Stepwise assembly of fibrin bilayers on self-assembled monolayers of alkanethiolates : Influence of surface chemistry. In: Journal of Physical Chemistry C. 2007 ; Vol. 111, No. 24. pp. 8504-8508.
@article{18431df488a74d5eb09036dafe8b71bd,
title = "Stepwise assembly of fibrin bilayers on self-assembled monolayers of alkanethiolates: Influence of surface chemistry",
abstract = "Fibrin bilayers are formed in a stepwise manner on four model surfaces: hydrophobic, neutral hydrophilic, positively charged, and negatively charged. These surfaces are self-assembled monolayers (SAMs) of alkanethiolates on gold with different terminal groups. A surface plasmon resonance (SPR) sensor is used to monitor the formation of fibrin bilayers in real time. The structures of the fibrin bilayers formed on SAMs of different surface chemistries are proposed, based on the amount of fibrinogen in each layer. The efficiency of the physically adsorbed fibrinogen to promote fibrin formation is assessed by the ratio of the amount of fibrinogen in the second layer to that in the first layer. It is shown for the first time that the orientation/conformation of an adsorbed fibrinogen layer determined by underlying surface chemistry affects its reactivity with soluble fibrinogen, resulting in fibrin bilayers with different molecular arrangements. Bovine aortic endothelial cells cultured on fibrin bilayers formed on CH 3- and OH- SAMs exhibited different morphologies and growth characteristics. Results show that fibrin bilayers with different molecular arrangements and thus different mechanochemical properties lead to different cellular responses.",
author = "Hua Wang and Ratner, {Buddy D.} and Sage, {E. Helene} and Shaoyi Jiang",
year = "2007",
month = "6",
day = "21",
doi = "10.1021/jp066960a",
language = "English (US)",
volume = "111",
pages = "8504--8508",
journal = "Journal of Physical Chemistry C",
issn = "1932-7447",
publisher = "American Chemical Society",
number = "24",

}

TY - JOUR

T1 - Stepwise assembly of fibrin bilayers on self-assembled monolayers of alkanethiolates

T2 - Influence of surface chemistry

AU - Wang, Hua

AU - Ratner, Buddy D.

AU - Sage, E. Helene

AU - Jiang, Shaoyi

PY - 2007/6/21

Y1 - 2007/6/21

N2 - Fibrin bilayers are formed in a stepwise manner on four model surfaces: hydrophobic, neutral hydrophilic, positively charged, and negatively charged. These surfaces are self-assembled monolayers (SAMs) of alkanethiolates on gold with different terminal groups. A surface plasmon resonance (SPR) sensor is used to monitor the formation of fibrin bilayers in real time. The structures of the fibrin bilayers formed on SAMs of different surface chemistries are proposed, based on the amount of fibrinogen in each layer. The efficiency of the physically adsorbed fibrinogen to promote fibrin formation is assessed by the ratio of the amount of fibrinogen in the second layer to that in the first layer. It is shown for the first time that the orientation/conformation of an adsorbed fibrinogen layer determined by underlying surface chemistry affects its reactivity with soluble fibrinogen, resulting in fibrin bilayers with different molecular arrangements. Bovine aortic endothelial cells cultured on fibrin bilayers formed on CH 3- and OH- SAMs exhibited different morphologies and growth characteristics. Results show that fibrin bilayers with different molecular arrangements and thus different mechanochemical properties lead to different cellular responses.

AB - Fibrin bilayers are formed in a stepwise manner on four model surfaces: hydrophobic, neutral hydrophilic, positively charged, and negatively charged. These surfaces are self-assembled monolayers (SAMs) of alkanethiolates on gold with different terminal groups. A surface plasmon resonance (SPR) sensor is used to monitor the formation of fibrin bilayers in real time. The structures of the fibrin bilayers formed on SAMs of different surface chemistries are proposed, based on the amount of fibrinogen in each layer. The efficiency of the physically adsorbed fibrinogen to promote fibrin formation is assessed by the ratio of the amount of fibrinogen in the second layer to that in the first layer. It is shown for the first time that the orientation/conformation of an adsorbed fibrinogen layer determined by underlying surface chemistry affects its reactivity with soluble fibrinogen, resulting in fibrin bilayers with different molecular arrangements. Bovine aortic endothelial cells cultured on fibrin bilayers formed on CH 3- and OH- SAMs exhibited different morphologies and growth characteristics. Results show that fibrin bilayers with different molecular arrangements and thus different mechanochemical properties lead to different cellular responses.

UR - http://www.scopus.com/inward/record.url?scp=34547270223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547270223&partnerID=8YFLogxK

U2 - 10.1021/jp066960a

DO - 10.1021/jp066960a

M3 - Article

AN - SCOPUS:34547270223

VL - 111

SP - 8504

EP - 8508

JO - Journal of Physical Chemistry C

JF - Journal of Physical Chemistry C

SN - 1932-7447

IS - 24

ER -